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首页> 外文期刊>Chromatographia >Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS
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Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS

机译：肺癌的代谢组学研究及放疗对肺癌患者的影响：超高性能HILIC结合Q-TOF MS分析高极性代谢物

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摘要

In this study, plasma samples were collected from 28 health volunteers and 66 lung cancer patients. Ultraperformance HILIC/Q-TOF MS-based metabonomic techniques were employed to compare the highly polar metabolites (HPMs) in the plasma of patients with lung cancer and those in the plasma of healthy volunteers. High separation efficiency and good repeatability of the HILIC/Q-TOF MS method were observed. Partial least squares discriminant analysis (PLS-DA) of the data from both ESI+ and ESI− mode revealed good classifications between the health volunteer and the lung cancer patients. This result showed that the HPMs from lung cancer patients and healthy controls were significantly different. The correct classification rate of models in both ion modes reached 100%, indicating that the metabonomics method established in this study could predict the plasma samples with high accuracy. Besides, we found 19 ions that show a significant difference in levels between lung cancer patients and healthy controls. Finally, we conducted a primary study of the effect of radiotherapy on HPMs in lung cancer patients. Clear differences among the HPMs of patients in different radiotherapy periods and significant changes in levels of the 19 ions after radiotherapy were noted. This demonstrated that radiotherapy influences the metabolism system of patients.
机译：在这项研究中，血浆样本是从28名健康志愿者和66名肺癌患者中收集的。基于超高性能HILIC / Q-TOF MS的代谢组学技术被用于比较肺癌患者血浆和健康志愿者血浆中的高极性代谢物（HPM）。观察到HILIC / Q-TOF MS方法具有较高的分离效率和良好的重复性。来自ESI +和ESI-模式的数据的偏最小二乘判别分析（PLS-DA）显示了健康志愿者和肺癌患者之间的良好分类。该结果表明，来自肺癌患者和健康对照者的HPM明显不同。两种离子模式下模型的正确分类率均达到100％，这表明本研究建立的代谢组学方法可以准确预测血浆样品。此外，我们发现19个离子在肺癌患者和健康对照之间显示出显着的水平差异。最后，我们对放疗对肺癌患者HPM的影​​响进行了初步研究。注意到在不同放疗期间患者的HPM之间存在明显差异，并且放疗后19个离子的水平发生了显着变化。这表明放疗会影响患者的代谢系统。

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