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首页> 外文期刊>Chromatographia >Off-line Coupling of Isotachophoresis and High Performance Liquid Chromatography for Determination of Flavonoids in Plant Extract
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Off-line Coupling of Isotachophoresis and High Performance Liquid Chromatography for Determination of Flavonoids in Plant Extract

机译：等速电泳和高效液相色谱离线耦合测定植物提取物中的类黄酮

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摘要

The use of isotachophoretic sample pretreatment coupled with high performance liquid chromatography for the analysis of some flavonoids occurring in plant extracts of Hypericum perforatum and Crataegus sp. is described. The samples were extracted with methanol by means of sonication in low temperature. The optimal leading electrolyte was used 10 mM Cl− as a leading ion in a buffer system at apparent pH*=7.2 (adjusted by TRIS) and terminating electrolyte was 50 mM boric acid at apparent pH*=8.2 (adjusted by barium hydroxide). The ITP electrolytes contained 20% (v/v) of methanol. To improve the sample pre-treatment, a pair of discrete ITP spacers defining the trapped constituents was used. Major components presented in the extracts were separated on a Discovery C18 and Discovery RP Amid C16 columns with a gradient mobile phase consisting of methanol, acetonitrile and diluted ortho-phosphoric acid. The quantification was performed by using external standards. The recoveries of the coupled ITP-HPLC analytical procedure were in the range of 91.2–95.6%.
机译：等速电泳样品预处理与高效液相色谱结合用于分析贯叶连翘和山楂属植物提取物中的某些类黄酮。描述。通过在低温下超声处理，用甲醇提取样品。最佳前导电解质在表观pH * = = 7.2（经TRIS调节）下以10 mM Cl-作为缓冲液中的前导离子，终止电解质为表观pH * <50的硼酸。 / sup> ＝ 8.2（通过氢氧化钡调节）。 ITP电解质包含20％（v / v）的甲醇。为了改进样品的预处理，使用了一对离散的ITP间隔基来定义被捕获的成分。萃取物中的主要成分在具有甲醇，乙腈和稀正磷酸组成的梯度流动相的Discovery C18和Discovery RP Amid C16色谱柱上进行分离。通过使用外部标准品进行定量。偶联的ITP-HPLC分析方法的回收率在91.2–95.6％之间。

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