Systematic gene targeting on the X chromosome of Drosophila melanogaster

Nicole Beinert; Meike Werner; Gordon Dowe; Ho-Ryun Chung; Herbert Jäckle; Ulrich Schäfer

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Systematic gene targeting on the X chromosome of Drosophila melanogaster

机译：系统基因靶向果蝇X染色体

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The genome of the model organism Drosophila melanogaster has been sequenced and annotated. Based on this groundwork, we performed a systematic genetic screen of the D. melanogaster X chromosome, which carries about one sixth of the genes of the organism. We generated a collection of single P-element insertions to provide genetic and molecular access to virtually all X-chromosomal genes. The study complements earlier work designed to systematically identify vital genes on the X chromosome by targeting transcription units which are phenotypically silent. We describe single UAS sequence-bearing P-element insertions throughout the X chromosome, which allows one to express the tagged genes under control of tissue/organ-directed GAL4 activity. In addition, the present collection of single insertion lines provides a tool to generate chromosomal deletions which are on average less than 33 kb in size.

机译：模式生物果蝇果蝇的基因组已测序和注释。基于这一基础，我们对D. melanogaster X染色体进行了系统的遗传筛选，该染色体携带了该生物的约六分之一的基因。我们生成了单个P元素插入的集合，以提供对几乎所有X染色体基因的遗传和分子访问。该研究通过设计针对表型沉默的转录单位，对旨在系统地鉴定X染色体上重要基因的早期工作进行了补充。我们描述了整个X染色体上单个带有UAS序列的P元素插入，它允许人们在组织/器官定向GAL4活性的控制下表达标记的基因。另外，本发明的单插入线的集合提供了产生平均大小小于33kb的染色体缺失的工具。

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《Chromosoma》 |2004年第6期|271-275|共5页
作者
Nicole Beinert; Meike Werner; Gordon Dowe; Ho-Ryun Chung; Herbert Jäckle; Ulrich Schäfer;
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Max-Planck-Institut für biophysikalische Chemie Abt. Molekulare Entwicklungsbiologie;

Max-Planck-Institut für biophysikalische Chemie Abt. Molekulare Entwicklungsbiologie;

Max-Planck-Institut für biophysikalische Chemie Abt. Molekulare Entwicklungsbiologie;

Max-Planck-Institut für biophysikalische Chemie Abt. Molekulare Entwicklungsbiologie;

Max-Planck-Institut für biophysikalische Chemie Abt. Molekulare Entwicklungsbiologie;

Max-Planck-Institut für biophysikalische Chemie Abt. Molekulare Entwicklungsbiologie;

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1. A New Resource for Characterizing X-Linked Genes in Drosophila melanogaster: Systematic Coverage and Subdivision of the X Chromosome With Nested, Y-Linked Duplications [J] . C. Adam Brown, Eric P. Spana, Jennifer A. Deal, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 2010,第4期

机译：表征果蝇X连锁基因的新资源：X染色体与嵌套的Y连锁重复的系统覆盖和细分。
2. Sequence signatures involved in targeting the male-specific lethal complex to X-chromosomal genes in Drosophila melanogaster [J] . Philge Philip, Fredrik Pettersson, Per Stenberg BMC Genomics . 2012,第1期

机译：在果蝇中将男性特异性致死复合物靶向X染色体基因的序列特征
3. P-Element Insertion Alleles of Essential Genes on the Third Chromosome of Drosophila melanogaster Correlation of Physical and Cytogenetic Maps in Chromosomal Region 86E-87F [J] . Charalambos Savakis, Christos Louis, David M. Glover, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1997,第4期

机译：果蝇第三个染色体上必需基因的P元素插入等位基因与86E-87F染色体区域物理图谱和细胞遗传图谱的相关性
4. Quantitative analysis of chromosome motion in Drosophila melanogaster [C] . Wallace F. Marshall, Univ. of California/San Francisco, San Francisco, Three-Dimensional Microscopy: Image Acquisition and Processing II . 1995

机译：果蝇染色体运动的定量分析
5. Chromosomal interactions and its impact on gene regulation: Transvection at the Men locus in Drosophila melanogaster. [D] . Lum, Thomas. 2011

机译：染色体相互作用及其对基因调控的影响：在果蝇中的男性基因座处进行平移。
6. A New Resource for Characterizing X-Linked Genes in Drosophila melanogaster: Systematic Coverage and Subdivision of the X Chromosome With Nested Y-Linked Duplications [O] . R. Kimberley Cook, Megan E. Deal, Jennifer A. Deal, 2010

机译：表征果蝇X连锁基因的新资源：X染色体与嵌套的Y连锁重复的系统覆盖和细分。
7. A New Resource for Characterizing X-Linked Genes in Drosophila melanogaster: Systematic Coverage and Subdivision of the X Chromosome With Nested, Y-Linked Duplications [O] . Cook, R. Kimberley, Deal, Megan E., Deal, Jennifer A., 2010

机译：表征果蝇X连锁基因的新资源：X染色体与嵌套的Y连锁重复的系统覆盖和细分。

Systematic gene targeting on the X chromosome of Drosophila melanogaster

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