...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Combinatorial Chemistry & High Throughput Screening >Generation of Anti-Colorectal Cancer Fab Phage Display Libraries With a High Percentage of Diverse Antigen-Reactive Clones
【24h】

Generation of Anti-Colorectal Cancer Fab Phage Display Libraries With a High Percentage of Diverse Antigen-Reactive Clones

机译：高比例的多种抗原反应性克隆的抗大肠癌Fab噬菌体展示文库的生成

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A combinatorial Fab phage display library was generated from the antibody variable region genes of each of 2 BALB/c mice immunized with the human colorectal cancer cell lines SW480, SW948, and SW837. These libraries were shown to be diverse by nucleotide sequencing and diagnostic restriction enzyme digestion (fingerprinting) of individual members. The two libraries were combined and selected for binding to a suspension of formaldehyde-fixed human colorectal cancer cells in two successive rounds of selection and phage amplification by infection of bacteria. Analysis of the selected libraries as well as individual library clones by ELISA, showed binding to the cancer cell lines in both formaldehyde-fixed and native forms. Fifty five percent and 94% of library clones were positive for colorectal cancer cell binding after the first and second rounds of selection, respectively. Fingerprinting of individual clones showed the first round selected library to be very diverse and the second round selected library to be of more limited diversity. After absorption with normal human cell types, these anti-cancer selected libraries could be used to develop therapeutic and/or diagnostic agents.
机译：从用人结肠直肠癌细胞系SW480，SW948和SW837免疫的2只BALB / c小鼠的抗体可变区基因生成组合Fab噬菌体展示文库。通过单个成员的核苷酸测序和诊断性限制性内切酶消化（指纹图谱）显示这些文库是多种多样的。合并两个文库，并选择在两个连续的选择和通过细菌感染的噬菌体扩增中与甲醛固定的人结肠直肠癌细胞的悬浮液结合。通过ELISA对所选文库以及单个文库克隆的分析显示，甲醛固定形式和天然形式均与癌细胞系结合。在第一轮和第二轮选择后，分别有55％和94％的文库克隆对结直肠癌细胞结合呈阳性。单个克隆的指纹显示，第一轮选择的文库非常多样化，第二轮选择的文库具有更有限的多样性。在被正常人类细胞类型吸收后，这些抗癌选择的文库可用于开发治疗剂和/或诊断剂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号