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Combinatorial Peptide Library for the Analysis of Antigen Recognition by T Cells

机译：用于T细胞识别抗原的组合肽库

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摘要

Peptides that consist of 19 residues with random sequences (X19) were considered to deliver antigenic stimuli to CD4T cells. When IL-4, IL-7, IL-9, IL-15 and agonistic Ab to CD29 were co-cultured with single peripheral CD4T cells in the presence of X19 and feeder cells, T cells exhibited clonal expansion. These T cell clones showed heterogeneous proliferation patterns against KGXXXXXXXXXGK-based and KGXXXXXXXXXGKGKK-based combinatorial peptide libraries. Pattern-match search on one of the T cell clones resulted in peptide ligand candidates, one of which induced proliferation, as did protein molecules carrying the corresponding sequence. Combinatorial chemistry was useful in determining not only peptide ligands but also peptide superagonists. For this purpose, use of reverse-phase hydrophobic interaction chromatography and mass spectrometry analysis was efficient. Detailed methods are described in the paper.
机译：由19个具有随机序列（X19）的残基组成的肽被认为可以将抗原刺激物传递给CD4T细胞。当在X19和饲养细胞的存在下，将IL-4，IL-7，IL-9，IL-15和激动剂对CD29的抗体与单个外周CD4T细胞共培养时，T细胞显示出克隆扩增。这些T细胞克隆显示针对基于KGXXXXXXXXXGK和基于KGXXXXXXXXXGKGKK的组合肽库的异质增殖模式。在T细胞克隆之一上进行模式匹配搜索会产生肽配体候选物，其中之一可诱导增殖，而带有相应序列的蛋白质分子也是如此。组合化学不仅可用于确定肽配体，而且还可用于确定肽超激动剂。为此目的，使用反相疏水相互作用色谱和质谱分析是有效的。本文描述了详细的方法。

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