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Alternative Bacteriophage Display Systems

机译:替代噬菌体展示系统

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摘要

Filamentous phage has been extensively used to implement various aspects of phage display technology. The success of these organisms as vectors to present foreign peptides and to link them to their coding sequences is a consequence of their structural and biological characteristics. Some of these properties, however, represent a limitation when one attempts to display proteins that cannot be efficiently exported through the bacterial membrane or do not fold properly in the periplasm. Thus, the desirability of developing alternative display systems was recognised recently and led to the development of a different class of display vectors that assemble their capsid in the cytoplasm and are released via cell lysis. This review describes and compares the properties of these alternative display systems.
机译:丝状噬菌体已被广泛用于实施噬菌体展示技术的各个方面。这些生物作为载体成功呈现外源肽并将其连接至其编码序列是其结构和生物学特性的结果。然而,当人们试图展示无法通过细菌膜有效输出或无法在周质中正确折叠的蛋白质时,其中一些特性会受到限制。因此,近来开发替代展示系统的需求得到认可,并导致了另一类展示载体的开发,这些展示载体将其衣壳组装在细胞质中并通过细胞裂解释放。这篇评论描述和比较了这些替代显示系统的属性。

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