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首页> 外文期刊>Crop and Pasture Science >A laboratory procedure for determining relative levels of field resistance in soybeans to Phytophthora megasperma f.sp. Glycinea
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A laboratory procedure for determining relative levels of field resistance in soybeans to Phytophthora megasperma f.sp. Glycinea

机译：一种测定大豆对大豆疫霉菌的相对抗性水平的实验室程序。甘氨酸

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摘要

A reliable laboratory assay is described which allows rapid determination of relative levels of field resistance in soybeans to Phytophthora megasperma fsp, glycinea. The expression of field resistance depended on both the inoculum level and the age of seedlings at time of saturation of the potting mix with water. At inoculum levels in the range 0.018-0.576 g dry weight of mycelium per kg dry weight of potting mix, ready differentiation in the resistance levels of the cultivars Davis (field resistant) and Semstar (field susceptible) could be made after 7 days of continuous saturation of the mix, which commenced 3 days after sowing. When inoculated by the conventional hypocotyl puncture technique, both cultivars gave susceptible reactions. The seedling assay was used to screen a range of soybean cultivars with known levels of field resistance to the fungus. Disease assessments made in the laboratory assay were similar to those expected under natural conditions of infection in the field. Possible use of the method in future soybean breeding programs is discussed.
机译：描述了一种可靠的实验室测定法，该测定法可以快速确定大豆中对巨大疫霉（Fytophthora megasperma fsp），甘氨酸的相对抗性水平。盆栽混合物与水饱和时，田间抗性的表达取决于接种量和幼苗的年龄。在接种量为每公斤盆栽混合物干重0.018-0.576 g菌丝干重的范围内，连续培养7天后，可以迅速区分出戴维斯（田间抗性）和Semstar（田间易感）品种的抗性水平混合物在播种后三天开始饱和。当通过常规的下胚轴穿刺技术接种时，两个品种均产生易感反应。幼苗测定法用于筛选具有已知水平的真菌抗性的一系列大豆品种。实验室测定中进行的疾病评估与现场自然感染条件下的预期评估相似。讨论了该方法在未来大豆育种计划中的可能用途。

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    《Crop and Pasture Science》 |1982年第1期|p.33-39|共7页
  • 作者

    JAG Irwin and PW Langdon;

  • 作者单位

    Queensland Department of Primary Industries, Plant Pathology Branch,Meiers Road, Indooroopilly, Qld 4068.;

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