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Abscisic Acid Suppression of Phenylalanine Ammonia-Lyase Activity and mRNA and Resistance of Soybeans to Phytophthora megasperma f.sp. glycinea

机译:脱落酸抑制苯丙氨酸解氨酶活性和mRNA以及大豆对巨大疫霉的抗性甘氨酸

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摘要

Etiolated hypocotyls of the resistant soybean (Glycine max [L.] Merr.) cultivar Harosoy 63 became susceptible to Phytophthora megasperma (Drechs.) f.sp. glycinea (Hildeb.) Kuan and Erwin race 1 after treatment with abscisic acid. Susceptibility was expressed by increases in lesion size and a major decrease in accumulation of the isoflavonoid phytoalexin, glyceollin. In untreated hypocotyls, activity of phenylalanine ammonia-lyase and accumulation of mRNA for this enzyme increased rapidly after infection, but these increases were suppressed in abscisic acid-treated hypocotyls. The results suggest the possibility that biosynthesis of glyceollin in the resistance response of soybeans may be controlled at the transcriptional level by changes in abscisic acid concentrations caused by infection.
机译:抗性大豆(Glycine max [L.] Merr。)栽培品种Harosoy 63的黄化胚轴对巨大疫霉(Drechsthora megasperma)(Drechs。f.sp.)敏感。脱落酸处理后,甘氨酸(Hildeb。)Kuan和Erwin第1种族。易感性通过病灶尺寸的增加和异黄酮植物抗毒素甘油糖醇的积累的显着减少来表示。在未经处理的下胚轴中,苯丙氨酸解氨酶的活性和该酶的mRNA积累在感染后迅速增加,但在脱落酸处理的下胚轴中抑制了这些增加。结果表明,通过感染引起的脱落酸浓度的变化,可以在转录水平上控制大豆抗逆反应中糖精蛋白的生物合成。

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