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首页> 外文期刊>Current Genetics >Development of a strain-specific SCAR marker for the detection of Trichoderma atroviride 11, a biological control agent against soilborne fungal plant pathogens
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Development of a strain-specific SCAR marker for the detection of Trichoderma atroviride 11, a biological control agent against soilborne fungal plant pathogens

机译：开发用于检测木霉阿特罗韦德11的菌株特异性SCAR标记物，该木霉是针对土壤传播的真菌植物病原体的生物防治剂

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摘要

The genus Trichoderma includes biocontrol agents (BCAs) effective against soilborne plant pathogenic fungi. Several potentially useful strains for biological control are difficult to distinguish from other strains of Trichoderma found in the field. So, there is a need to find ways to monitor these strains when applied to natural pathosystems. We have used random amplified polymorphic DNA (RAPD) markers to estimate genetic variation among sixteen strains of the species T. asperellum, T. atroviride, T. harzianum, T. inhamatum and T. longibrachiatum previously selected as BCAs, and to obtain fingerprinting patterns. Analysis of these polymorphisms revealed four distinct groups, in agreement with previous studies. Some of the RAPD products generated were used to design specific primers. Diagnostic PCR performed using these primers specifically identify the strain T. atroviride 11, showing that DNA markers may be successfully used for identification purposes. This SCAR (sequence-characterised amplified region) marker can clearly distinguish strain 11 from other closely related Trichoderma strains.
机译：木霉属包括对土壤传播的植物致病真菌有效的生物防治剂（BCA）。难以将几种可能用于生物防治的菌株与本领域发现的其他木霉菌株区分开。因此，有必要找到一种方法来监测应用于自然病理系统的这些菌株。我们已经使用随机扩增多态性DNA（RAPD）标记来估计先前选择为BCA的16种曲霉菌，曲霉，阿特罗韦德，哈茨木霉，烟曲霉和长曲霉的16个菌株之间的遗传变异，并获得指纹图谱。对这些多态性的分析揭示了四个不同的组，与先前的研究一致。生成的一些RAPD产品用于设计特异性引物。使用这些引物进行的诊断PCR可特异性鉴定A.trotroide毒株11，表明DNA标记可成功用于鉴定目的。该SCAR（序列特征性扩增区域）标记可以清楚地区分菌株11与其他密切相关的木霉菌株。

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  • 来源
    《Current Genetics》 |2001年第6期|343-350|共8页
  • 作者

    M. Rosa Hermosa; Isabel Grondona; José María Díaz-Mínguez; Enrique A. Iturriaga; Enrique Monte;

  • 作者单位

    Departamento de Microbiología y Genética Lab. 208 Edificio Departamental Universidad de Salamanca Avenida Campo Charro s 37007 Salamanca Spain e-mail: emv@gugu.usal.es;

    Departamento de Microbiología y Genética Lab. 208 Edificio Departamental Universidad de Salamanca Avenida Campo Charro s 37007 Salamanca Spain e-mail: emv@gugu.usal.es;

    Departamento de Microbiología y Genética Lab. 208 Edificio Departamental Universidad de Salamanca Avenida Campo Charro s 37007 Salamanca Spain e-mail: emv@gugu.usal.es;

    Departamento de Microbiología y Genética Lab. 208 Edificio Departamental Universidad de Salamanca Avenida Campo Charro s 37007 Salamanca Spain e-mail: emv@gugu.usal.es;

    Departamento de Microbiología y Genética Lab. 208 Edificio Departamental Universidad de Salamanca Avenida Campo Charro s 37007 Salamanca Spain e-mail: emv@gugu.usal.es;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Key wordsTrichoderma; Biocontrol agents; Molecular markers; SCARs;

    机译：关键词木霉;生物防治剂;分子标记;SCAR;

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