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Effect of Plant Age on Endophytic Bacterial Diversity of Balloon Flower (Platycodon grandiflorum) Root and Their Antimicrobial Activities

机译:种植年龄对桔梗桔梗内生细菌多样性及其抗菌活性的影响

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摘要

Balloon flower (Platycodon grandiflorum) is widely cultivated vegetable and used as a remedy for asthma in East Asia. Experiments were conducted to isolate endophytic bacteria from 1-, 3-, and 6-year-old balloon flower roots and to analyze the enzymatic, antifungal, and anti-human pathogenic activities of the potential endophytic biocontrol agents obtained. Total 120 bacterial colonies were isolated from the interior of all balloon flower roots samples. Phylogenetic analysis based on 16S rRNA gene sequences showed that the population of ‘low G + C gram-positive bacteria’ (LGCGPB) gradually increased 60.0–80.0% from 1 to 6 years balloon flower sample. On the other hand, maximum hydrolytic enzyme activity showing endophytic bacteria was under LGCGPB, among the bacterial strains, Bacillus sp. (BF1-1 and BF3-8), Bacillus sp. (BF1-2 and BF3-5), and Bacillus sp. (BF1-3, BF3-6, and BF6-4) showed maximum enzyme activities. Besides, Bacillus licheniformis (BF3-5 and BF6-6) and Bacillus pumilus (BF6-1) showed maximum antifungal activity against Phytophthora capsici, Fusarium oxysporum, Rhizoctonia solani, and Pythium ultimum. Moreover, Bacillus licheniformis was found in 3 and 6 years balloon flower roots, but Bacillus pumilus was found only in 6 years sample. It is presumed that older balloon flower plants invite more potential antifungal endophytes for there protection from plant diseases. In addition, Bacillus sp. (BF1-2 and BF3-5) showed maximum anti-human pathogenic activity. So, plant age is presumed to influence diversity of balloon flower endophytic bacteria.
机译:桔梗花(Platycodon grandiflorum)是一种广泛种植的蔬菜,在东亚被用作治疗哮喘的药物。进行了实验,从1岁,3岁和6岁的桔梗花根中分离出内生细菌,并分析了获得的潜在内生生物防治剂的酶促,抗真菌和抗人致病活性。从所有桔梗花根样品的内部分离出总共120个细菌菌落。根据16S rRNA基因序列的系统发育分析表明,“低G + C革兰氏阳性细菌”(LGCGPB)的种群从1到6年的桔梗花样本逐渐增加了60.0–80.0%。另一方面,在细菌菌株芽孢杆菌中,显示出内生细菌的最大水解酶活性在LGCGPB之下。 (BF1-1和BF3-8),芽孢杆菌属。 (BF1-2和BF3-5)和芽孢杆菌(Bacillus sp。) (BF1-3,BF3-6和BF6-4)显示最大的酶活性。此外,地衣芽孢杆菌(BF3-5和BF6-6)和短小芽孢杆菌(BF6-1)对辣椒疫霉菌,尖孢镰刀菌,茄根霉和终腐霉菌显示出最大的抗真菌活性。此外,地衣芽孢杆菌在3年和6年的桔梗花根中被发现,而短小芽孢杆菌仅在6年的样品中被发现。据推测,较老的桔梗花植物会招募更多潜在的抗真菌内生菌,以保护植物免受疾病侵害。另外,芽孢杆菌属。 (BF1-2和BF3-5)显示出最大的抗人致病活性。因此,推测植物年龄会影响桔梗花内生细菌的多样性。

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