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ISSR-PCR fingerprinting of Ukrainian sweet cherry (Prunus avium L.) cultivars

机译：乌克兰甜樱桃品种的ISSR-PCR指纹图谱

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摘要

ISSR-PCR markers were used to assess genetic diversity and to elucidate relatedness among 21 Ukrainian and three West-European sweet cherry cultivars that are widely cultivated in Ukraine. The discriminatory potential was tested for 11 ISSR-PCR primers, which produced 193 amplicons. UBC 835, 836, 841, and 881 were identified as the best primers suitable for routine application. The studied cultivars appear to be genetically highly heterogenic and can be divided into two main groups. The first one includes closely related cultivars obtained by hybridization of Drogans gelbe Knorpelkirsche, Valerii Chkalov, and some other forms. The second group comprises less similar cultivars derived from several West-European and unknown ancestors. Origin of several Ukrainian cultivars is discussed.
机译：ISSR-PCR标记用于评估遗传多样性并阐明21个乌克兰和三个在乌克兰广泛种植的西欧甜樱桃品种之间的相关性。测试了11种ISSR-PCR引物的识别潜力，该引物产生了193个扩增子。 UBC 835、836、841和881被确定为适合常规应用的最佳引物。研究的品种似乎在遗传上具有高度异质性，可以分为两个主要类别。第一个包括紧密相关的品种，这些品种是通过将德罗甘斯·凝胶·克诺佩尔基什切，瓦莱里·奇卡洛夫和其他一些形式杂交获得的。第二组包括不太相似的品种，这些品种来自几个西欧和未知的祖先。讨论了几种乌克兰品种的起源。

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