...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cytology and genetics >Role of genes snoaM, snoaL, and snoaE in the biosynthesis of nogalamycin in Streptomyces nogalater Lv65
【24h】

Role of genes snoaM, snoaL, and snoaE in the biosynthesis of nogalamycin in Streptomyces nogalater Lv65

机译：snoaM，snoaL和snoaE基因在Nogalater Lv65链霉菌生物素合成中的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bioinformatic analysis of snoaL, snoaM, and snoaE genes from Streptomyces nogalater, a microorganism producing the anthracycline antibiotic nogalamycin, is reported. The results of phylogenetic analysis demonstrated high similarity of SnoaL, SnoaM, and SnoaE proteins to cyclases involved in the biosynthesis of various antibiotics. Insertional inactivation of polyketide cyclase genes in the chromosome of S. nogalater Lv65 was performed, and S. nogalater strains MI, LI, and EI were thus obtained. Inactivation of the above named genes did not affect the morphologic characteristics of S. nogalater. The inability of strains MI, LI, and EI to synthesize nogalamycin was demonstrated. Antibiotic synthesis was restored when extra copies of snoaL, snoaM, and snoaE genes were introduced into the above named strains. Streptomycete strains obtained in the present work, as well as nogalamycin cyclase genes, can be used in combinatorial biosynthesis of anthracyclin antibiotics.
机译：据报道，来自产链霉菌Nogalater的微生物snoaL，snoaM和snoaE基因的生物信息学分析，该微生物产生蒽环类抗生素诺古霉素。系统发育分析的结果表明，SnoaL，SnoaM和SnoaE蛋白与参与各种抗生素生物合成的环化酶高度相似。进行了S.nogalater Lv65染色体上聚酮化合物环化酶基因的插入失活，从而获得了S. nogalater菌株MI，LI和EI。上述基因的失活不影响S. nogalater的形态特征。证实了菌株MI，LI和EI不能合成诺加霉素。将多余拷贝的snoaL，snoaM和snoaE基因导入上述菌株后，抗生素合成得以恢复。在本工作中获得的链霉菌菌株以及诺加霉素环化酶基因可用于蒽环素抗生素的组合生物合成。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号