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首页> 外文期刊>Cytology and genetics >Effects of Tyrosine Kinase and Phosphatase Inhibitors on Mitosis Progression in Synchronized Tobacco BY-2 Cells
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Effects of Tyrosine Kinase and Phosphatase Inhibitors on Mitosis Progression in Synchronized Tobacco BY-2 Cells

机译：酪氨酸激酶和磷酸酶抑制剂对同步烟草BY-2细胞有丝分裂进程的影响

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To test whether reversible tubulin phosphorylation plays any role in the process of plant mitosis the effects of inhibitors of tyrosine kinases, herbimycin A, genistein and tyrphostin AG 18, and of an inhibitor of tyrosine phosphatases, sodium orthovanadate, on microtubule organization and mitosis progression in a synchronized BY-2 culture has been investigated. It was found that treatment with inhibitors of tyrosine kinases of BY-2 cells at the G_2/M transition did not lead to visible disturbances of mitotic microtubule structures, while it did reduce the frequency of their appearance. We assume that a decreased tyrosine phosphorylation level could alter the microtubule dynamic instability parameters during interphase/prophase transition. All types of tyrosine kinase inhibitors used caused a prophase delay: herbimycin A and genistein for 2 h, and tyrphostin AG 18 for 1 h. Thereafter the peak of mitosis was displaced for 1 h by herbimycin A or genistein exposure, but after tyrphostin AG 18 treatment the timing of the mitosis-peak was comparable to that in control cells. Enhancement of tyrosine phosphorylation induced by the tyrosine phosphatase inhibitor resulted in the opposite effect on BY-2 mitosis transition. Culture treatment with sodium orthovanadate during 1 h resulted in an accelerated start of the prophase and did not lead to the alteration in time of the mitotic index peak formation, as compared to control cells. We suppose that the reversible tyrosine phosphorylation can be involved in the regulation of interphase to M phase transition possible through regulation of microtubule dynamics in plant cells.
机译：为了测试可逆性微管蛋白的磷酸化在植物有丝分裂过程中是否发挥任何作用，酪氨酸激酶，除草素A，染料木黄酮和酪氨酸磷酸酶AG 18抑制剂以及酪氨酸磷酸酶，原钒酸钠对微管组织和有丝分裂进程的影响已经研究了同步的BY-2文化。发现在G_2 / M过渡期用BY-2细胞的酪氨酸激酶抑制剂处理不会导致有丝分裂微管结构的可见紊乱，尽管确实减少了它们出现的频率。我们假设酪氨酸磷酸化水平降低可能会在相间/前相过渡过程中改变微管动态不稳定性参数。所使用的所有类型的酪氨酸激酶抑制剂都会导致前期延迟：除草霉素A和染料木黄酮2 h，酪氨酸激酶AG 18 1 h。此后，通过除草霉素A或染料木黄酮暴露使有丝分裂的峰移位1小时，但是在酪氨酸蛋白酶抑制剂AG 18处理后，有丝分裂高峰的时间与对照细胞中的峰时间相当。酪氨酸磷酸酶抑制剂诱导的酪氨酸磷酸化增强导致对BY-2有丝分裂过渡的相反作用。与对照细胞相比，用原钒酸钠进行的培养处理在1小时内可加速前期的开始，并且不会导致有丝分裂指数峰形成时间的改变。我们认为可逆酪氨酸磷酸化可通过调节植物细胞中微管的动力学来参与相间至M相转变的调节。

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  • 来源
    《Cytology and genetics》 |2012年第5期|263-271|共9页
  • 作者

    Ya. A. Sheremet; A. I. Yemets; A. Azmi; K. Vissenberg; J.-P. Verbelen; Ya. B. Blume;

  • 作者单位

    Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev;

    Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev;

    University of Antwerp, Plant Growth and Development, Biology Department, Belgium;

    University of Antwerp, Plant Growth and Development, Biology Department, Belgium;

    University of Antwerp, Plant Growth and Development, Biology Department, Belgium;

    Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev;

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