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首页> 外文期刊>Cytology and genetics >Effect of Serine/Threonine Protein Kinases and Protein Phosphatases Inhibitors on Mitosis Progression in a Synchronized Tobacco BY-2 Culture
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Effect of Serine/Threonine Protein Kinases and Protein Phosphatases Inhibitors on Mitosis Progression in a Synchronized Tobacco BY-2 Culture

机译：丝氨酸/苏氨酸蛋白激酶和蛋白磷酸酶抑制剂对同步烟草BY-2培养中有丝分裂进程的影响

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摘要

In order to investigate the role of various serine/threonine protein kinases and protein phosphatases in the regulation of mitosis progression in plant cells, the influence of cyclin- (olomoucine) and Ca~(2+)/calmodulin-dependent (W7) protein kinases inhibitors, as well as protein kinase C inhibitors (H7 and staurosporine), and a protein phosphatases inhibitor (okadaic acid) on mitosis progression in synchronized tobacco BY-2 cells has been studied. It was found that BY-2 culture treatment with inhibitors of cyclin-dependent protein kinases and protein kinase C caused a prophase delay, reduced the mitotic index, and displaced the mitotic peak as compared with control cells. Inhibition of Ca~(2+)/calmodulin-dependent protein kinases enhanced the cells entry into prophase and delayed their exit from mitosis. Meanwhile inhibition of serine/threonine protein phosphatases insignificantly enhances synchronized BY-2 cells entering into all phases of mitosis.
机译：为了研究各种丝氨酸/苏氨酸蛋白激酶和蛋白磷酸酶在调节植物细胞有丝分裂进程中的作用，以及细胞周期蛋白-（olomoucine）和Ca〜（2 +）/钙调蛋白依赖性（W7）蛋白激酶的影响对同步烟草BY-2细胞中有丝分裂进程的蛋白抑制剂，蛋白激酶C抑制剂（H7和星形孢菌素）和蛋白磷酸酶抑制剂（冈田酸）进行了研究。发现与细胞周期蛋白相比，用细胞周期蛋白依赖性蛋白激酶和蛋白激酶C抑制剂进行的BY-2培养处理引起前期延迟，降低了有丝分裂指数并置换了有丝分裂峰。 Ca〜（2 +）/钙调蛋白依赖性蛋白激酶的抑制作用增强了细胞进入前期并延迟了它们从有丝分裂的退出。同时，丝氨酸/苏氨酸蛋白磷酸酶的抑制作用微不足道地增强了同步BY-2细胞进入有丝分裂的所有阶段。

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  • 来源
    《Cytology and genetics》 |2012年第2期|p.89-95|共7页
  • 作者

    Ya. A. Sheremet; A. I. Emets; A. Azmi; K. Vissenberg; J.-P. Verbelen; Ya. B. Blume;

  • 作者单位

    Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev, Ukraine;

    Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev, Ukraine;

    University of Antwerp, Antwerpen, Belgium;

    University of Antwerp, Antwerpen, Belgium;

    University of Antwerp, Antwerpen, Belgium;

    Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev, Ukraine;

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