Generation of Transgenic Barley Lines Producing Human Lactoferrin using Mutant Alpha-Tubulin Gene as the Selective Marker

I. V. Tanasienko; A. I. Yemets; Y. V. Pirko; V. I. Korhkovyy; N. Abumhadi; Ya. B. Blume

首页> 外文期刊>Cytology and genetics >Generation of Transgenic Barley Lines Producing Human Lactoferrin using Mutant Alpha-Tubulin Gene as the Selective Marker

【24h】

Generation of Transgenic Barley Lines Producing Human Lactoferrin using Mutant Alpha-Tubulin Gene as the Selective Marker

机译：使用突变的α-管蛋白基因作为选择标记的生产人乳铁蛋白的转基因大麦品系的产生

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The biolistic transformation method was used for genetic improvement of three commercial cultivars of barley (Oksamytoviy, Vodogray, and Hetman). The plasmid pHLFTuBA containing target gene h LFencoding human lactoferrin under the control of the rice glutein B-1 promoter GluB-1 was used for transformation. The gene encoding mutant alfa-tubulin conferring resistance to trifluralin (dinitroaniline herbicide) was used as the selective marker. The screening of different trifluralin concentrations ranging from 0.1-30 μM was used for determination of selective concentration of the agent. Two transgenic barley lines of cultivars Oksamytoviy and Hetman's callus line were selected after 2-3 months of cultivation on 10 uM of trifluralin. To confirm stable integration of the transformed gene, the PCR analysis of leafs from regenerated plant after their adaptation on the ground was carried out. The 734 bp fragment of the target gene was amplified from both regenerated plants.

机译：生物弹转化法用于三种大麦商业品种（Oksamytoviy，Vodogray和Hetman）的遗传改良。在水稻叶黄素B-1启动子GluB-1的控制下，将包含编码人乳铁蛋白的靶基因h LF的质粒pHLFTuBA用于转化。编码赋予三氟拉林（二硝基苯胺除草剂）抗性的突变型α-微管蛋白的基因用作选择标记。筛选范围从0.1-30μM的不同氟乐灵用于确定药物的选择性浓度。在10uM的氟乐灵上培养2-3个月后，选择了两个Okkamytoviy品种和Hetman愈伤组织的转基因大麦品系。为了确认转化基因的稳定整合，对再生植物的叶片在地面适应后进行了PCR分析。从两种再生植物中扩增靶基因的734bp片段。

著录项

来源
《Cytology and genetics》 |2011年第1期|p.1-6|共6页
作者
I. V. Tanasienko; A. I. Yemets; Y. V. Pirko; V. I. Korhkovyy; N. Abumhadi; Ya. B. Blume;
展开▼
作者单位

Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev, Ukraine;

Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev, Ukraine;

Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev, Ukraine;

Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev, Ukraine;

Agro Bio Institute, Sofia, Bulgaria;

Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev, Ukraine;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. Generation of human lactoferrin transgenic cloned goats using donor cells with dual markers and a modified selection procedure [J] . An Li-You, Yuan Yu-Guo, Yu Bao-Li, Theriogenology . 2012,第6期

机译：使用具有双重标记的供体细胞和改良的选择程序生成人乳铁蛋白转基因克隆山羊
2. Transgenic ginseng cell lines that produce high levels of a human lactoferrin. [J] . Kwon SY, Jo SH, Lee OS, Planta medica: Natural products and medicinal plant research . 2003,第11期

机译：产生高水平人乳铁蛋白的转基因人参细胞系。
3. Rapid Generation of Barley Mutant Lines With High Nitrogen Uptake Efficiency by Microspore Mutagenesis and Field Screening [J] . Runhong Gao, Guimei Guo, Chunyan Fang, Frontiers in Plant Science . 2018,第4期

机译：通过小孢子诱变和田间筛选快速产生具有高氮吸收效率的大麦突变体
4. The "Italian" Barley Genetic Mutant Collection: Conservation, Development of New Mutants and Use [C] . Stanca Antonio Michele, Tumino Giorgio, Pagani Donata, Advance in barley sciences : proceedings of 11th international barley genetics symposium . 2012

机译：“意大利”大麦遗传突变体收藏：保存，开发新突变体和使用
5. The lactoferrin receptor mediates lactoferrin endocytosis and is involved in the effects of lactoferrin on intestinal proliferation and characterization of the human lactoferrin receptor gene promoter. [D] . Jiang, Rulan. 2010

机译：乳铁蛋白受体介导乳铁蛋白的内吞作用，并参与乳铁蛋白对人乳铁蛋白受体基因启动子的肠道增殖和特征的影响。
6. Rapid Generation of Barley Mutant Lines With High Nitrogen Uptake Efficiency by Microspore Mutagenesis and Field Screening [O] . Runhong Gao, Guimei Guo, Chunyan Fang, -1

机译：通过小孢子诱变和田间筛选快速产生具有高氮吸收效率的大麦突变体
7. Generation of Five Human Lactoferrin Transgenic Cloned Goats Using Fibroblast Cells and Their Methylation Status of Putative Differential Methylation Regions of IGF2R and H19 Imprinted Genes [O] . Meng, L., Wan, Y., Sun, Y., 2013

机译：使用成纤维细胞生成五种人类乳铁蛋白转基因克隆山羊及其IGF2R和H19印迹基因推定甲基化差异区域的甲基化状态

Generation of Transgenic Barley Lines Producing Human Lactoferrin using Mutant Alpha-Tubulin Gene as the Selective Marker

摘要

著录项

相似文献

相关主题

期刊订阅