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Polypurine/Polypyrimidine Sequences with the Potential of Forming Triplexes in the Proviral DNA of Bovine Retroviruses

机译：在牛逆转录病毒的原病毒DNA中具有形成三链体潜力的多嘌呤/多嘧啶序列

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摘要

The perfect interstrand triplexes that could potentially arise in the proviral DNA of two widespread cattle retroviruses such as bovine leukemia virus (BLV) and bovine immunodeficiency virus (BIV) were determined. The fragments, which formed triplexes at acidic pH, were found in the genomes of both viruses; five fragments were found in BVL and 10 fragments in BIV. One of these fragments (it is localized in the BVL gag gene) might exist like a part of a cruciform structure. Existence of the triplexes was experimentally confirmed by visualization of supercoiled pGEMEX DNA with the use of atomic force microscopy; six fragments with mirror symmetry, which are necessary for formation of intramolecular triplexes, were found. Triplexes represent one of the elements of the signaling mechanisms of the genome function. Maps of triplex location in the cattle retroviral genome were built.
机译：确定了可能在两种广泛的牛逆转录病毒（如牛白血病病毒（BLV）和牛免疫缺陷病毒（BIV））的原病毒DNA中潜在出现的完美链间三链体。在两种病毒的基因组中均发现了在酸性pH下形成三链体的片段。在BVL中发现5个片段，在BIV中发现10个片段。这些片段之一（位于BVL gag基因中）可能像十字形结构的一部分一样存在。通过使用原子力显微镜对超螺旋pGEMEX DNA进行可视化实验，证实了三元组的存在。发现了六个具有镜面对称性的片段，这是形成分子内三链体所必需的。三元组代表基因组功能的信号传导机制的要素之一。建立了牛逆转录病毒基因组中三链体定位的图。

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    《Cytology and genetics》 |2010年第1期|P.7-13|共7页
  • 作者

    O. Yu. Limanskaya;

  • 作者单位

    Mechnikov Institute of Microbiology and Immunology, Academy of Medical Sciences of Ukraine, Kharkov, 61057 Ukraine National Scientific Centre 'Institute of Experimental and Clinical Veterinary Medicine', ul. Pushkinskaya 83, Kharkov, 61023 Ukraine';

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