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Salmine and the Homeotic Integrity of Early Embryos of Norway Spruce

机译：丹参和挪威云杉早期胚的同源完整性

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摘要

Salmine, an arginine-rich protamine, is explored for its concentration-dependent potential to restructure the genome and remodel the homeotic development of Norway spruce embryos expressing monozy-gotic cleavage polyembryony (MCP). In controls and at low salmine, two protein fractions on SDS-PAGE gels were associated with cells responsible for generating the basal plan for early embryagenesis. With high salmine, embryonal initials no longer differentiated into embryonal tubes. Embryos having embryonal tubes no longer enucleated and differentiated into embryonal suspensors. Biomass and amino acid N declined. Nuclear and cytoplas-mic organization was disrupted and nucleoli were highly vacuolated. The transcription of the two protein fractions, PCNA (cyclin) activity and MCP were blocked. Cellular proteins were turned over by proteasomal ubiquitination and others released into the culture medium. Biomass loss and gluconeogenesis of amino acids led to the accumulation of free arginine N. No evidence was obtained with salmine for the remodeling of cells into gametes.
机译：人们探索了富含精氨酸的鱼精蛋白Salmine，它具有浓度依赖性的潜力，可重组基因组并重塑表达单核-卵裂多胚性（MCP）的挪威云杉胚的同源发育。在对照中和低盐胺含量下，SDS-PAGE凝胶上的两个蛋白质部分与负责产生早期胚胎发生基础计划的细胞有关。有了高含量的氨氮，胚胎的首字母就不再分化成胚管了。具有胚管的胚不再去核并分化成胚悬液。生物量和氨基酸N下降。核和细胞质组织被破坏，核仁高度空泡化。 PCNA（cyclin）活性和MCP这两个蛋白质部分的转录被阻止。细胞蛋白通过蛋白酶体泛素化而被翻转，其他蛋白被释放到培养基中。氨基酸的生物量损失和糖异生导致游离精氨酸N的积累。利用氨氮无法获得将细胞重塑成配子的证据。

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  • 来源
    《Cytology and genetics》 |2010年第2期|67-78|共12页
  • 作者

    D. J. Durzan;

  • 作者单位

    Plant Sciences MS 6, 204 Old Davis Rd, University of California, Davis, CA, 95616;

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