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首页> 外文期刊>Cytology and genetics >Development of Specific and Degenerate Primers for CesA Genes Encoding Cellulose Synthase in Flax (Linum Usitatissimum L.)
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Development of Specific and Degenerate Primers for CesA Genes Encoding Cellulose Synthase in Flax (Linum Usitatissimum L.)

机译：亚麻中纤维素合酶编码CesA基因的特异性和简并引物的开发（Linum Usitatissimum L.）

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摘要

Representatives of the CesA multigene family that control the synthesis of the catalytic subunits of the cellulose synthase complex were described for a number of higher plants. It has been established that the HVR2 region of these genes is class-specific and determines the involvement of the gene product in the synthesis of either the primary or secondary cell wall. The purpose of the current research was to develop degenerate and specific primers for parts of the CesA genes to allow the construction of molecular markers for the class-specific HVR2 region. Two pairs of specific primers for the CesA-1 and CesA-6 genes as well as a pair of degenerate primers for the HVR2 region of all flax CesA genes were developed, based on analysis of the CesA ESTs as well as the full-length cDNA sequences of the CesA genes in Arabidopsis, poplar, maize, and cotton that are available in the GenBank. Fragments of the expected size were amplified using flax cDNA as a template (201 bp for CesA-1, 300 bp for CesA-6, and 600 bp for HVR2). The markers developed in this research can be used for CesA gene cloning and sequencing, analysis of gene copy numbers as well as characterization of tissue- and development specific gene expression.
机译：对于许多高等植物，描述了控制纤维素合酶复合物催化亚基合成的CesA多基因家族的代表。已经确定这些基因的HVR2区域是类特异性的，并确定基因产物参与原代或次要细胞壁的合成。当前研究的目的是为部分CesA基因开发简并引物，以构建针对特定类HVR2区域的分子标记。基于对CesA EST和全长cDNA的分析，开发了两对用于CesA-1和CesA-6基因的特异性引物，以及一对用于所有亚麻CesA基因的HVR2区的简并引物。 GenBank中可用的拟南芥，白杨，玉米和棉花中CesA基因的序列。使用亚麻cDNA作为模板扩增预期大小的片段（CesA-1为201 bp，CesA-6为300 bp，HVR2为600 bp）。在这项研究中开发的标记可用于CesA基因克隆和测序，基因拷贝数分析以及组织和发育特异性基因表达的表征。

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  • 来源
    《Cytology and genetics》 |2010年第4期|p.195-199|共5页
  • 作者

    Z. E. Grushetskaya; V. A. Lemesh; L. V. Khotyleva;

  • 作者单位

    Institute of Genetics and Cytology, Academy of Sciences of Belarus, Minsk, Belarus;

    Institute of Genetics and Cytology, Academy of Sciences of Belarus, Minsk, Belarus;

    Institute of Genetics and Cytology, Academy of Sciences of Belarus, Minsk, Belarus;

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