Production of Human Interferon ALPHA 2b in Plants of Nicotiana excelsior by Agrobacterium-Mediated Transient Expression

Y. R. Sindarovska; I. M. Gerasymenko; Y. V. Sheludko; Z. M. Olevinskaya; N. Y. Spivak; N. V. Kuchuk

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Production of Human Interferon ALPHA 2b in Plants of Nicotiana excelsior by Agrobacterium-Mediated Transient Expression

机译：农杆菌介导的瞬时表达在精油烟草中产生人干扰素ALPHA 2b

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Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with TV. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 × 103 IU/g fresh weight (FW) with an average of 2.1 ±0.8 × 10~3 IU/gFW. It proves that TV. excelsior is a suitable host for Agrobacterium-mediated transient expression of genes encoding physiologically active human proteins. The transient expression con ditions optimized for GFP marker protein were confirmed to be preferable for hlFN α2b.

机译：人干扰素α2b基因在极好的烟草植物中瞬时表达。与电视融合。用于改善质外体靶向性并在优化条件下进行表达的洋李叶钙化蛋白信号肽的干扰素活性最高为3.2×103 IU / g鲜重（FW），平均为2.1±0.8×10〜3 IU / gFW。证明了电视。 excelsior是农杆菌介导的编码生理活性人蛋白基因的瞬时表达的合适宿主。证实针对GFP标记蛋白优化的瞬时表达条件对于hIFNα2b是优选的。

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《Cytology and genetics》 |2010年第5期|p.313-316|共4页
作者
Y. R. Sindarovska; I. M. Gerasymenko; Y. V. Sheludko; Z. M. Olevinskaya; N. Y. Spivak; N. V. Kuchuk;
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Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Zabolotnogo str. 148, Kiev, 03680, Ukraine;

rnInstitute of Cell Biology and Genetic Engineering NAS of Ukraine, Zabolotnogo str. 148, Kiev, 03680, Ukraine;

rnInstitute of Cell Biology and Genetic Engineering NAS of Ukraine, Zabolotnogo str. 148, Kiev, 03680, Ukraine;

rnInstitute of Microbiology and Virology NAS of Ukraine, Zabolotnogo str. 154, Kiev, 03680, Ukraine;

rnInstitute of Microbiology and Virology NAS of Ukraine, Zabolotnogo str. 154, Kiev, 03680, Ukraine;

rnInstitute of Cell Biology and Genetic Engineering NAS of Ukraine, Zabolotnogo str. 148, Kiev, 03680, Ukraine;

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1. Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures [J] . Biotechnology Letters . 2017,第7期

机译：合成冷诱导型启动子在尼古拉尼灭绝的尼古拉氏菌介导的瞬时表达期间增强重组蛋白质积累在寒冷的温度下
2. Cloning, Transformation and Expression of Human Interferon α2b Gene in Tobacco Plant (Nicotiana tabacum cv. xanthi) [J] . Hamid Galehdari, Hamid Rajabi-Memari, Khalil Alamisaied, Jundishapur Journal of Natural Pharmaceutical Products . 2012,第3期

机译：人干扰素α2b基因在烟草植物中的克隆，转化及表达
3. Production of Human Alpha 2b Interferon in E.coli Expression System [J] . Nehzat Abdolrasouli, Hamid Rajabi Memari, Mohammad Roayaei Ardakani, Jundishapur Scientific Medical Journal . 2013,第3期

机译：大肠杆菌表达系统中人α2b干扰素的生产
4. Reducing Batch-to-Batch Variability of Agrobacterium-Mediated Transient Protein Expression In Plant Tissue Culture [C] . Jeffrey S. Larsen, Wayne R. Curtis American Institute of Chemical Engineers Annual Meeting . 2008

机译：降低农杆菌介导的植物组织培养中介导的瞬态蛋白表达的批量变异性
5. Agrobacterium-mediated transient protein expression in plant cell suspension and root cultures [D] . Collens, Jason I. 2006

机译：农杆菌介导的植物细胞悬浮液和根系培养物中瞬时蛋白的表达
6. Cloning Transformation and Expression of Human Interferon α2b Gene in Tobacco Plant (Nicotiana tabacum cv. xanthi) [O] . Shahrzad Ahangarzadeh, Mohammad Hosein Daneshvar, Hamid Rajabi-Memari, 2012

机译：人干扰素α2b基因在烟草植物中的克隆转化及表达
7. Production of human interferon alfa 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression [O] . Sindarovska Y.R., Gerasymenko I.M., Sheludko Y.V., 2010

机译：农杆菌介导的瞬时表达在优良烟草中产生人干扰素α2b

Production of Human Interferon ALPHA 2b in Plants of Nicotiana excelsior by Agrobacterium-Mediated Transient Expression

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