Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 • 10³ IU/g fresh weight (FW) with an average of 2.1 ± 0.8 • 10³ IU/g FW. It proves that N. excelsior is a suitable host for Agrobacterium-mediated transient expression of genes encoding physiologically active human proteins. The transient expression conditions optimized for GFP marker protein were confirmed to be preferable for hIFN α2b.
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机译：人干扰素α2b基因在极好的烟草植物中瞬时表达。与李子叶猪笼草钙网蛋白信号肽融合以改善质外体靶向性，并在优化条件下进行表达，产生的干扰素最大活性为3.2•10³IU / g鲜重（FW），平均为2.1±0.8•10³IU / g FW 。证明了N.excelsior是农杆菌介导的编码生理活性人蛋白基因的瞬时表达的合适宿主。证实了针对GFP标记蛋白优化的瞬时表达条件对于hIFNα2b是优选的。
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