Identification of Allele Variants of Cattle Milk Productivity Genes Using PCR and the Anti-Primer Method

A. N. Rashydov; V. G. Spiridonov; O. N. Konoval; M. D. Melnychuk

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Identification of Allele Variants of Cattle Milk Productivity Genes Using PCR and the Anti-Primer Method

机译：PCR和反引物方法鉴定牛乳生产力基因的等位基因变异

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In this study, we present two independent approaches in using Real-Time PCR for the detection of single nucleotide polymorphism (SNP) in genes encoding a kappa-casein and an acyl CoA: diacylglycerol acyltransferase 1 (DGAT1) in cattle. The samples from 296 Ukrainian-bred dairy cows were analyzed. The genotype frequencies for the kappa-casein gene were as follows: AA, 0.58; AB, 0.34; and BB, 0.08; the fre quencies for the acyl-CoA: diacylglycerol acyltransferase 1 gene were as follows: AA, 0.7; AK, 0.26; and KK, 0.04. The high efficiency of the anti-primer method was shown. The anti-primer Real-Time PCR test takes only 2-2.5 h and allows for the complete identification of the unknown allelic variant of the studied genes.

机译：在这项研究中，我们提出了两种独立的方法，使用实时PCR检测编码Kappa-酪蛋白和酰基CoA的基因中的单核苷酸多态性（SNP）：牛中的二酰基甘油酰基转移酶1（DGAT1）。分析了来自296头乌克兰奶牛的样品。 Kappa-酪蛋白基因的基因型频率如下：AA，0.58； Ab，0.58。 AB，0.34；和BB，0.08；酰基辅酶A：二酰基甘油酰基转移酶1基因的频率如下：AA，0.7； AK 0.26；和KK，0.04。显示了抗引物方法的高效率。抗引物实时荧光定量PCR检测仅需2-2.5小时，可以完全鉴定所研究基因的未知等位基因变体。

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《Cytology and genetics》 |2010年第5期|p.272-275|共4页
作者
A. N. Rashydov; V. G. Spiridonov; O. N. Konoval; M. D. Melnychuk;
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Ukrainian Laboratory of Quality and Safety of Agricultural Products, National University of Life and Environmental Sciences of Ukraine, vul. Heroiv Oborony 15, Kyiv, 03041 Ukraine;

rnUkrainian Laboratory of Quality and Safety of Agricultural Products, National University of Life and Environmental Sciences of Ukraine, vul. Heroiv Oborony 15, Kyiv, 03041 Ukraine;

rnUkrainian Laboratory of Quality and Safety of Agricultural Products, National University of Life and Environmental Sciences of Ukraine, vul. Heroiv Oborony 15, Kyiv, 03041 Ukraine;

rnNational University of Life and Environmental Sciences of Ukraine, vul. Heroiv Oborony 15, Kyiv, 03041 Ukraine;

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Identification of Allele Variants of Cattle Milk Productivity Genes Using PCR and the Anti-Primer Method

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