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首页> 外文期刊>Cytology and genetics >PCR Analysis of the Wheat Varieties and Near-Isogenic Wheat Lines with the Use of Allele-Specific Primers for the Gli-1 and Glu-3 Loci
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PCR Analysis of the Wheat Varieties and Near-Isogenic Wheat Lines with the Use of Allele-Specific Primers for the Gli-1 and Glu-3 Loci

机译：使用等位基因特异性引物对Gli-1和Glu-3基因座进行小麦品种和近等基因小麦品系的PCR分析

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摘要

The allelic characteristics of Gli-A1, Gli-B1, Gli-D1 and Glu-A3 loci of 14 bread wheat varieties and 6 near-isogenic wheat lines derived from the Bezosta 1 variety were found by the use of PCR. The conformity between molecular-genetic and storage protein electrophoretic data was revealed: the Glia1.2 allele corresponds to the Gli-Alo and Gli-Alm allelic variants of gliadin blocks; the GliA1.1 PCR allele corresponds to the Gli-Alf Gli-Alb and Gli-Alc variants of gliadin blocks; the GliB1.1 allele corresponds to the Gli-B1b and Gli-B1d allelic variants; and the GliB1.2 allele corresponds to the Gli-Ble, Gli-Blg and Gli-Blc variants. A new PCR allele with primers for marker GliB1. 1 at the Gli-Bl locus in the GLI-B1-12 line (with the gliadin Gli-Blo block), which was generated from crossing of Bezosta 1 and the variety Levent, was detected.
机译：通过PCR发现了14个面包小麦品种和6个近等基因小麦品系的Gli-A1，Gli-B1，Gli-D1和Glu-A3基因座的等位基因特征。揭示了分子遗传学和贮藏蛋白电泳数据之间的一致性：Glia1.2等位基因对应于醇溶蛋白嵌段的Gli-Alo和Gli-Alm等位基因变体。 GliA1.1 PCR等位基因对应于醇溶蛋白嵌段的Gli-Alf Gli-Alb和Gli-Alc变体; GliB1.1等位基因对应于Gli-B1b和Gli-B1d等位基因变体； GliB1.2等位基因对应于Gli-Ble，Gli-Blg和Gli-Blc变体。带有标记GliB1引物的新PCR等位基因。检测到在GLI-B1-12系的Gli-B1基因座处的1（具有麦醇溶蛋白Gli-Blo嵌段），其是由Bezosta 1与品种Levent的杂交产生的。

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  • 来源
    《Cytology and genetics》 |2010年第6期|p.345-353|共9页
  • 作者

    A.M.Polishchuk; S.V.Chebotar; E.M.Blagodarova; N.A.Kozub; I.A.Sozinov; Yu.M.Sivolap;

  • 作者单位

    South Plant Biotechnology Center, Ukrainian Academy of Agrarian Sciences, str. Ovidiopolska Doroga 3, Odessa, 65036, Ukraine;

    South Plant Biotechnology Center, Ukrainian Academy of Agrarian Sciences, str. Ovidiopolska Doroga 3, Odessa, 65036, Ukraine;

    The Plant Breeding and Genetics Institute - National Center of Seed and Cultivar Investigation, Ukrainian Academy of Agrarian Sciences, str. Ovidiopolska Doroga 3, Odessa, 65036, Ukraine;

    Institute of Plant Protection, Ukrainian Academy of Agrarian Sciences, str. Vasyl'kivs 'ka 33, Kyiv, 03022, Ukraine,DU 'Institute of Food Biotechnology and Genomics, Ukrainian National Academy of Sciences, str. Osypovs'kogo 2A, Kyiv, 04123, Ukraine;

    DU 'Institute of Food Biotechnology and Genomics, Ukrainian National Academy of Sciences, str. Osypovs'kogo 2A, Kyiv, 04123, Ukraine;

    South Plant Biotechnology Center, Ukrainian Academy of Agrarian Sciences, str. Ovidiopolska Doroga 3, Odessa, 65036, Ukraine;

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