MOLECULAR GENETIC ANALYSIS OF WHEAT (Triticum aestivum L.) GENOME WITH INTROGRESSION OF Aegilops cylindrica Host GENETIC ELEMENTS

A. V. Gagaev; Yu. M. Sivolap

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MOLECULAR GENETIC ANALYSIS OF WHEAT (Triticum aestivum L.) GENOME WITH INTROGRESSION OF Aegilops cylindrica Host GENETIC ELEMENTS

机译：小麦（Triticum aestivum L.）基因组的分子遗传分析与导入的拟南芥（Aegilops cylindrica）寄主遗传元件

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Wheat-jointed goatgrass hybrids Triticum aestivum L. (2n = 42) x Aegilops cylindrica Host (2n = 28) were investigated by means of microsatellite markers. In two BC_1F_9 lines changes were detected in the genome, which were related to loss of individual DNA fragments of the initial wheat variety or appearance of jointed goatgrass genomic elements. Furthermore, in the investigated plants were noted new amplicons not described in the parents. Substitutions of homoeologous jointed goatgrass chromosomes for bread wheat chromosomes were not found. A microsatellite analysis of BC_2F_5 plants showed the stable incorporation of jointed goatgrass genetic elements into wheat plants, elimination of individual transferred sections of the jointed goatgrass genome during backcrossing, and decrease in the size of the introgression fragment during repeated backcrossing. Introgressive bread wheat lines were classified according to genomic changes.

机译：利用微卫星标记研究了小麦连接的山羊草杂种小麦（2n = 42）x埃及古山羊草寄主（2n = 28）。在两个BC_1F_9品系中检测到基因组变化，这些变化与最初小麦品种的单个DNA片段的丢失或接合的山羊草基因组元件的出现有关。此外，在所研究的植物中注意到亲本中未描述的新扩增子。没有发现用同源节理的山羊草染色体替代面包小麦染色体。对BC_2F_5植物的微卫星分析显示，将联合的山羊草遗传元件稳定地掺入小麦植株中，回交期间消除了联合的山羊草基因组的各个转移部分，并在重复的回交过程中减少了渗入片段的大小。根据基因组变化对渐进型面包小麦品系进行分类。

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《Cytology and genetics》 |2005年第3期|p.48-56|共9页
作者
A. V. Gagaev; Yu. M. Sivolap;
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作者单位

Southern Biotechnology Center in the Plant Industry, Ukrainian Academy of Sciences and Ukrainian Ministry of Education and Science, Ovidiopolskaya Doroga 3, Odessa, 65036;

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正文语种 eng
中图分类普通生物学;
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机译：Aegilops cylindrica Host×Triticum aestivum L. hybrids的早期子代中来自A，B和D基因组的小麦DNA标记渗入
2. Introgression of an imidazolinone-resistance gene from winter wheat (Triticum aestivum L.) into jointed goatgrass (Aegilops cylindrica Host) [J] . Perez-Jones Alejandro, Mallory-Smith Carol, Hansen Jennifer, Theoretical and Applied Genetics: International Journal of Breeding Research and Cell Genetics . 2006,第1期

机译：冬小麦（Triticum aestivum L.）的咪唑啉酮抗性基因渗入节理的山羊草（Aegilops cylindrica Host）
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4. Isolation and phylogenetic analysis of protein phosphatase 2C genes from wheat (Triticum aestivum L.) mature embryos. [C] . S. Nakamura, M. Chono, F. Abe, International Wheat Genetics Symposium . 2003

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5. Jointed goatgrass (Aegilops cylindrica Host) genetic diversity and hybridization with wheat (Triticum aestivum L.). [D] . Gandhi, Harish Tulshiramji. 2006

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机译：遗传对比拼写（Triticum aestivum ssp。spelta）种中α-麦醇溶蛋白表达基因的分子多样性并与面包小麦（T. aestivum ssp。aestivum）和相关二倍体小麦（Triticum）和埃及菊科（Aegilops）种进行比较
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MOLECULAR GENETIC ANALYSIS OF WHEAT (Triticum aestivum L.) GENOME WITH INTROGRESSION OF Aegilops cylindrica Host GENETIC ELEMENTS

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