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首页> 外文期刊>Cytology and genetics >The Use of a Micronucleus Test in Pinus pallasiana D. Don and Picea abies (L.) Karst. for the Assessment of Technogenic Pollution's Influence
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The Use of a Micronucleus Test in Pinus pallasiana D. Don and Picea abies (L.) Karst. for the Assessment of Technogenic Pollution's Influence

机译：微核试验在Palus pallasiana D. Don和Picea abies（L.）Karst中的使用。技术污染影响评估

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摘要

Results of the study on the frequency of micronuclei (MN) in the meristem of Pinus pallasiana D. Don and Picea abies (L.) Karst. seedlings from natural populations and plantings of technogenically polluted territories are presented. The level of cells with MN was 0.95 +/- 0.13% in the control P. pallasiana population. An increase in the level of cells with MN was observed under introduction- and technogenic-pollution conditions (2.41-3.38%). Similar results were shown for P. abies. The pathological cell contained a different amount of MN (from one to two for P. pallasiana and from one to six for P. abies). The size of MN varied from 0.13 to 14.93% of the diameter of the main nuclei in P. pallasiana cells and from 0.07 to 61.87% in P. abies cells. Multinucleated cells and cells with an irregular-shaped nucleus were observed. Correlation dependence between cytogenetic disorders and MN frequency (r = 0.98) was established. The use of MN of seedlings of studied species for the assessment of the genotoxicity of technogenically polluted areas was proposed.
机译：研究结果显示，Palus pallasiana D. Don和Picea abies（L.）Karst的分生组织中的微核（MN）频率。介绍了自然种群的幼苗和技术污染地区的种植园。在对照P.pallasiana种群中，MN的细胞水平为0.95 +/- 0.13％。在引入污染和技术污染条件下（2.41-3.38％）观察到MN细胞的水平增加。研究显示了类似的结果。病理细胞含有不同数量的MN（P。pallasiana从1到2，而A. abies从1到6）。 MN的大小在P.pallasiana细胞中为主核直径的0.13％至14.93％，在P.abis细胞中为0.07％至61.87％。观察到多核细胞和具有不规则形状核的细胞。建立了细胞遗传疾病和MN频率之间的相关性依赖性（r = 0.98）。提出了利用被研究物种的幼苗的MN来评估技术污染区域的遗传毒性。

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