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首页> 外文期刊>Cytology and genetics >Gene Expression Profiling during Life Cycle of Potato (Solanum tuberosum) Tubers by Microarray
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Gene Expression Profiling during Life Cycle of Potato (Solanum tuberosum) Tubers by Microarray

机译：基因芯片分析马铃薯（Solanum tuberosum）块茎生命周期中的基因表达谱

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摘要

Microarray was used to investigate the gene expression profile of potato tubers during its complete life cycle. The potato life cycle was divided into 8 different stages and total RNA was isolated and used as probe after labeling of cDNA generated from RNA samples. cDNAs were used to hybridize with potato microarray. Results of this study revealed that there is upregulation of many genes during developing stages (3b, 3c, 3d, 4a, 4b) compared to mature stages (6, 7). Many of those genes regulate growth, storage of protein and sugar, defense, inhibition of various proteinases, and oxidation. Some genes showed upregulation during certain growth stage (4a) compared to other growth stages, whereas, other genes showed upregulation in mature stages (stage 8). Profiling of gene expression during potato life cycle is essential step for enhancing potato for some highly economic traits like tuber nutrition quality, resistance to diseases, or using tubers as source for proteinase inhibitors. Also, this allows for isolation of some genes for biotechnology applications like proteinase inhibitors and improving nutrition and industrial value of tubers.
机译：微阵列用于研究马铃薯块茎整个生命周期的基因表达谱。马铃薯的生命周期分为8个不同阶段，在标记了RNA样品产生的cDNA后，将总RNA分离并用作探针。 cDNA用于与马铃薯微阵列杂交。这项研究的结果表明，与成熟阶段（6、7）相比，在发育阶段（3b，3c，3d，4a，4b）有许多基因上调。这些基因中的许多基因调节生长，蛋白质和糖的存储，防御，各种蛋白酶的抑制和氧化。与其他生长阶段相比，某些基因在某些生长阶段（4a）表现出上调，而其他基因在成熟阶段（阶段8）表现出上调。在马铃薯生命周期中进行基因表达分析对于增强马铃薯的某些高度经济性状（如块茎营养质量，抗病性或使用块茎作为蛋白酶抑制剂的来源）是必不可少的步骤。而且，这允许分离用于生物技术应用的某些基因，例如蛋白酶抑制剂，并改善块茎的营养和工业价值。

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