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首页> 外文期刊>Cytology and genetics >Comparative Analysis of Paddlefish (Polyodon spathula) Populations' Genetic Structure with Regard to Microsatellite DNA Markers
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Comparative Analysis of Paddlefish (Polyodon spathula) Populations' Genetic Structure with Regard to Microsatellite DNA Markers

机译:Paddlefish(Polyodon Spathula)群体对微卫星DNA标记的遗传结构的比较分析

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摘要

Comparative characteristics in genetic structure of cultured Ukrainian and Polish populations and the natural paddlefish populations from the United States is reported for three microsatellite DNA markers: Psp21, Psp26, and Psp28. The average Na value was 6.1 and 5.5 for the Ukrainian and Polish populations, respectively. The Na value for the natural populations was almost two times higher at 11.1 alleles on average. The mean values of observed (Ho) heterozygosity were shown to exceed those of expected heterozygosity (He) for both Ukrainian (0.709 0.616) and Polish (0.809 0.699) populations. The mean Ho and He values for the natural populations were in the range of genetic equilibrium according to the Hardy-Weinberg equation at 0.817 and 0.813, respectively. According to the data presented, changes in the total number of allelic variants in the cultured populations as compared to natural populations were demonstrated. The heterozygosity level values obtained and negative values of the fixation index Fis for the cultured populations point at the absence of inbreeding at the present stage of paddlefish cultivation, and this is indicative of a sufficient number of breeders with a heterozygous genotype for reproduction under aquaculture conditions.
机译:据报道,三种微卫星DNA标记:PSP21,PSP26和PSP28,据报道了乌克兰和波兰人群遗传结构的比较特征,以及美国的天然桨鱼种群。乌克兰和波兰人口的平均值为6.1和5.5。平均11.1等位基因的天然群体的Na值差不多两倍。观察到(HO)杂合性的平均值被显示超过乌克兰(0.709> 0.616)和抛光(0.809> 0.699)种群的预期杂合子(HE)。根据0.817和0.813的Hardy-Weinberg方程,自然群体的平均HO和HE值在遗传均衡范围内。根据呈现的数据,证明了与天然群体相比培养群体中的等位基因变体总数的变化。获得的杂合子水平值和在培养的培养群体的固定指标FIS的阴性值,并且在毛细胞培养的目前缺乏繁殖的情况下,这表明在水产养殖条件下具有足够数量的繁殖基因型进行繁殖基因型。

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