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首页> 外文期刊>Cytology and genetics >Development of an Effective In Vitro Regeneration System for Ukrainian Breeding Winter Rape Brassica napus L
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Development of an Effective In Vitro Regeneration System for Ukrainian Breeding Winter Rape Brassica napus L

机译：乌克兰繁殖冬季强奸芸苔湖湖有效的体外再生系统的开发

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摘要

An optimized regeneration method for commercial winter rape of theBn1line was proposed. Hypocotyl fragments of 6-day-old seedlings were used as explants. Regeneration occurred via organogenesis on an MS medium supplemented with 3 mg/L of 6-benzylaminopurine and 2 mg/L of 2-isopentyladenine. All obtained regenerated plants successfully formed roots on hormone-free media and were adapted to the soil conditions. The vernalization conditions were specified to promote budding and flowering at the level of 83.93 +/- 5.33%. The PCR analysis using the ISSR 2 and ISSR 15 markers has shown that no somaclonal variations inBn1winter rape occur under the protocols based on the proposed methodology. Thus, the developed methodology is efficient and economically profitable for obtaining biotechnology-based winter rape plants.
机译：提出了一种用于BNN1LLIL的商业冬季强奸的优化再生方法。 6日龄幼苗的幼杆子片段用作外植体。通过有机组织在补充有3mg / L的6-苄氨基嘌呤和2mg / L的2-异戊基腺嘌呤的MS培养基上发生再生。所有获得的再生植物在无激素培养基上成功形成根部，并适应土壤条件。核化条件被规定为促进萌芽和开花，在83.93 +/- 5.33％的水平。使用ISSR 2和ISSR 15标记的PCR分析表明，基于所提出的方法，在协议下没有发生inbn1winter强奸的糖髓变化。因此，开发的方法是用于获得基于生物技术的冬季强奸植物的有效且经济地有利可图。

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  • 来源
    《Cytology and genetics》 |2020年第4期|341-346|共6页
  • 作者

    Hnatyuk I. S.; Varchenko O. I; Kuchuk M. V; Parii M. F.; Symonenko Yu V;

  • 作者单位

    Natl Acad Sci Ukraine Inst Cell Biol & Genet Engn UA-03143 Kiev Ukraine|Ukrainian Sci Inst Plant Breeding UA-03022 Kiev Ukraine;

    Natl Acad Sci Ukraine Inst Cell Biol & Genet Engn UA-03143 Kiev Ukraine|Ukrainian Sci Inst Plant Breeding UA-03022 Kiev Ukraine;

    Natl Acad Sci Ukraine Inst Cell Biol & Genet Engn UA-03143 Kiev Ukraine;

    Ukrainian Sci Inst Plant Breeding UA-03022 Kiev Ukraine|Natl Univ Life & Environm Sci Ukraine UA-03041 Kiev Ukraine;

    Natl Acad Sci Ukraine Inst Cell Biol & Genet Engn UA-03143 Kiev Ukraine|Ukrainian Sci Inst Plant Breeding UA-03022 Kiev Ukraine;

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  • 正文语种 eng
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  • 关键词

    winter rape; regeneration; organogenesis; growth regulators; vernalization; somaclonal variability;

    机译：冬季强奸;再生;有机组织;生长调节因素;春化;索拉蒙可变性;

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