Application of Beta-Glucuronidase Transient Expression for Selection of Maize Genotypes Competent for Genetic Transformation

Nitovska I. O.; Abraimova O. Ye.; Duplij V. P.; Derkach K. V.; Satarova T. M.; Rudas V. A.; Cherchel V. Yu.; Dziubetskyi B. V.; Morgun B. V.

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Application of Beta-Glucuronidase Transient Expression for Selection of Maize Genotypes Competent for Genetic Transformation

机译：β-葡萄糖醛酸糖酶瞬时表达在选择玉米基因型选择遗传转化中的应用

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Genetic transformation of inbred maize lines and F-1 hybrids registered in Ukraine has been carried out. The study employed a biolistic method for genetic transformation of immature maize embryos that formed callus tissue and the pAHC25 vector containing the genes of phosphinothricin-N-acetyltransferase (bar) and beta-glucuronidase (uidA). As a result of the transformation of callus tissue of maize genotypes, lines resistant to phosphinothricin and regenerated plants were obtained. The activity of beta-glucuronidase in herbicide-resistant calli was detected. The presence of the bar gene in callus DNA was demonstrated by the PCR method. The rate of stable transformation ranged from 2.2 to 30% depending on the genotype. The relationship between the results of transient expression of the beta-glucuronidase gene and stable genetic transformation was observed. The proposed protocol for genetic transformation of maize using the study of transient expression of the beta-glucuronidase gene makes it possible to significantly simplify the process of selecting genotypes competent for genetic transformation and create transgenic organisms with new traits.

机译：已经进行了在乌克兰注册的自交式玉米系和F-1杂种的遗传转化。该研究采用了形成愈伤组织和含有磷素-N-乙酰转移酶（BAR-葡糖醛酸酯酶（UIDA）基因的愈伤组织和PAHC25载体的未成熟玉米胚胎遗传转化的生物化方法。由于玉米基因型的愈伤组织组织的转化，得到了对膦腈和再生植物的含量。检测到β-葡糖醛酸酶在除草剂耐药中的活性。通过PCR方法证明了愈伤组织DNA中的Bar基因的存在。根据基因型，稳定变换的速率范围为2.2至30％。观察到β-葡糖醛酸糖苷酶基因瞬时表达结果与稳定遗传转化的关系。使用β-葡糖醛酸酶基因的瞬时表达研究的玉米遗传转化的提出的遗传转化方案使得可以显着简化选择遗传转化的竞争力的基因型的过程，并用新的特征产生转基因生物。

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来源
《Cytology and genetics》 |2019年第6期|451-458|共8页
作者
Nitovska I. O.; Abraimova O. Ye.; Duplij V. P.; Derkach K. V.; Satarova T. M.; Rudas V. A.; Cherchel V. Yu.; Dziubetskyi B. V.; Morgun B. V.;
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作者单位

Natl Acad Sci Ukraine Inst Cell Biol & Genet Engn UA-03143 Kiev Ukraine;

Natl Acad Agr Sci Ukraine Inst Grain Crops UA-49600 Dnipro Ukraine;

Natl Acad Sci Ukraine Inst Cell Biol & Genet Engn UA-03143 Kiev Ukraine;

Natl Acad Agr Sci Ukraine Inst Grain Crops UA-49600 Dnipro Ukraine;

Natl Acad Agr Sci Ukraine Inst Grain Crops UA-49600 Dnipro Ukraine;

Natl Acad Sci Ukraine Inst Cell Biol & Genet Engn UA-03143 Kiev Ukraine;

Natl Acad Agr Sci Ukraine Inst Grain Crops UA-49600 Dnipro Ukraine;

Natl Acad Agr Sci Ukraine Inst Grain Crops UA-49600 Dnipro Ukraine;

Natl Acad Sci Ukraine Inst Cell Biol & Genet Engn UA-03143 Kiev Ukraine;

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正文语种 eng
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关键词
Zea mays L; immature embryos; biolistic genetic transformation; bar; uidA;

机译：Zea mays L;未成熟的胚胎;生物遗传转化;酒吧;UIDA;

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2. Transient beta-glucuronidase expression in lily (Lilium longflorum L.) pollen via wounding-assisted Agrobacterium-mediated transformation [J] . Kim SS, Shin DI, Park HS Biotechnology Letters . 2007,第6期

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3. Transient expression of the beta-glucuronidase gene in tissues of Arabidopsis thaliana by bombardment-mediated transformation. [J] . Seki M, Iida A, Morikawa H Molecular biotechnology . 1999,第3期

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机译：耐盐性提高的玉米基因型根中液泡型Na〜+ / H〜+反向转运蛋白（ZmNHX）和Na〜+的表达
5. Selection response, genetic variation, and combining ability of high oil maize genotypes. [D] . Mejaya, I-Made Jana. 2003

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6. Acetosyringone, pH and temperature effects on transient genetic transformation of immature embryos of Brazilian wheat genotypes by Agrobacterium tumefaciens [O] . Ernandes Manfroi, Elene Yamazaki-Lau, Magali F. Grando, 2020

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7. Amplification and expression of the beta-glucuronidase gene in maize plants by vectors based on maize streak virus [O] . Wen-Hui Shen, Barbara Hohn 1994

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Application of Beta-Glucuronidase Transient Expression for Selection of Maize Genotypes Competent for Genetic Transformation

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