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首页> 外文期刊>Biomarker Insights >Analysis of Lung Flute-collected Sputum for Lung Cancer Diagnosis:
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Analysis of Lung Flute-collected Sputum for Lung Cancer Diagnosis:

机译：肺痰收集痰对肺癌的诊断分析：

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Molecular analysis of sputum can help diagnose lung cancer. We have demonstrated that Lung Flute can be used to collect sputum from individuals who cannot spontaneously expectorate sputum. The objective of this study is to further evaluate the performance of the Lung Flute by comparing the characteristics of parallel samples collected with and without the Lung Flute and the usefulness for diagnosis of lung cancer. Fifty-six early-stage lung cancer patients (40 current smokers and 16 former smokers) and 73 cancer-free individuals (52 current smokers and 21 former smokers) were instructed to spontaneously cough and use Lung Flute for sputum sampling. Sputum cytology and polymerase chain reaction analysis of three miRNAs (miRs-21, 31, and 210) were performed in the specimens. All 92 current smokers and 11 (28.7%) of 37 former smokers spontaneously expectorated sputum and also produced sputum when using the Lung Flute. Twenty-seven former smokers (70.3%) who could not spontaneously expectorate sputum, however, were able to produce sputum when using the Lung Flute. The specimens were of low respiratory origin without contamination from other sources, eg, saliva. There was no difference of sputum volume and cell populations, diagnostic efficiency of cytology, and analysis of the miRNAs in the specimens collected by the two approaches. Analysis of the sputum miRNAs produced 83.93% sensitivity and 87.67% specificity for identifying lung cancer. Therefore, sputum collected by the Lung Flute has comparable features as spontaneously expectorated sputum. Using the Lung Flute enables former smokers who cannot spontaneously expectorate to provide adequate sputum to improve sputum collection for lung cancer diagnosis.
机译：痰液的分子分析可以帮助诊断肺癌。我们已经证明，肺长笛可用于收集无法自发排痰的人的痰液。这项研究的目的是通过比较有无肺脏长笛收集的平行样本的特征以及对肺癌诊断的实用性来进一步评估肺长笛的性能。指示56例早期肺癌患者（40名现吸烟者和16名以前吸烟者）和73名无癌个体（52名现吸烟者和21名以前吸烟者）自发咳嗽并使用肺长笛进行痰液采样。在标本中对三种miRNA（miRs-21、31和210）进行了痰细胞学检查和聚合酶链反应分析。目前所有92位吸烟者和37位前吸烟者中的11位（占28.7％）在使用肺长笛时会自发痰液并产生痰液。然而，不能自发排痰的二十七名前吸烟者（70.3％）在使用肺长笛时能够产生痰。标本是低呼吸源的，没有其他来源的污染，例如唾液。两种方法收集的标本中的痰量和细胞数量，细胞学诊断效率以及分析miRNA均无差异。痰中miRNA的分析可产生83.93％的敏感性和87.67％的特异性，可用于鉴定肺癌。因此，肺长笛收集的痰液具有与自发痰液相似的功能。使用肺长笛可使无法自发排痰的前吸烟者提供足够的痰液，以改善痰液收集以诊断肺癌。

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