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首页> 外文期刊>Bioengineered >Adaptive laboratory evolution of Klebsiella pneumoniae for improving 2,3-butanediol production
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Adaptive laboratory evolution of Klebsiella pneumoniae for improving 2,3-butanediol production

机译：肺炎克雷伯菌对改善2,3-丁二醇生产的适应性实验室进化

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摘要

Microbial production of 2,3-butanediol is limited by the toxic components in the lignocellulose hydrolysate. To improve the 2,3-butanediol production via Klebsiella pneumoniae from cotton stalk hydrolysate, a method coupling a high tolerance of strain and detoxification of the hydrolysate was thus investigated in this study. The strain tolerance of K. pneumoniae to the cotton stalk hydrolysate was improved via an adaptive laboratory evolution, which involved a stepwise increase in the hydrolysate concentration in the medium. Compared with the initial strain, the resulting strain increased the biomass 3.2-fold in a medium of 20?g/L hydrolysate and produced 10.45 g/L of 2,3-butanediol at an optimal concentration of 60 g/L hydrolysate. After detoxification of cotton stalk hydrolysate, the cell metabolism of K. pneumoniae was further promoted, and the 2,3-butanediol production increased by 1.2 folds. Using fed-batch fermentation, the concentration of 2,3-butanediol reached 35.5 g/L with a yield of 0.43 g/g. The results demonstrated that the bioconversion of low-cost cotton stalk hydrolysate into 2,3-butanediol improves the economics of microbial 2,3-butanediol production.
机译：2,3-丁二醇的微生物生产受到木质纤维素水解产物中有毒成分的限制。为了改善由棉秆水解产物通过肺炎克雷伯菌产生的2，3-丁二醇，在本研究中研究了结合高耐受性和水解产物解毒的方法。 K的应变容限。通过适应性实验室改进，改善了棉茎水解产物的肺炎，这涉及逐步增加培养基中水解产物的浓度。与初始菌株相比，所得菌株在20？g / L水解产物的培养基中使生物量增加了3.2倍，并以60 g / L的最佳水解浓度生产了10.45 g / L的2,3-丁二醇。棉秆水解产物解毒后，钾的细胞代谢。进一步促进了肺炎，并且2,3-丁二醇的产量增加了1.2倍。使用分批补料发酵，2,3-丁二醇的浓度达到35.5 g / L，产率为0.43 g / g。结果表明，低成本的棉秆水解产物生物转化为2，3-丁二醇可提高微生物2，3-丁二醇生产的经济性。

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