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Formation of supported bacterial lipid membrane mimics

机译：支持的细菌脂质膜模拟物的形成

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In recent years, a large effort has been spent on advancing the understanding of how surface-supported membranes are formed through vesicle fusion. The aim is to find simple model systems for investigating biophysical and biochemical interactions between constituents of cell membranes and, for example, drugs and toxins altering membrane function. Designing and controlling the self-assembly of model membranes onto sensor substrates thus constitutes an important field of research, enabling applications in, e.g., drug screening, dynamic biointerfaces, artificial noses, and research on membrane-active antibiotics. The authors have developed and investigated the formation of strongly negatively charged supported lipid membranes which systematically mimic bacterial membrane composition on three important biosensor materials: SiO2, TiO2, and indium tin oxide. By tuning the electrostatic interaction through balancing the lipid vesicle charge with the ionic strength of Ca2+ as a fusion promoter, the authors have optimized the self-assembly and obtained new insights into the details of lipid vesicle-surface interaction. The results will be useful for future development and application of specialized lipid membrane surface coatings prepared from complex lipid compositions. The adsorption processes were characterized by a quartz crystal microbalance with dissipation monitoring, optical waveguide lightmode spectroscopy, and fluorescence recovery after photobleaching, which allowed the determination of formation also of nonplanar supported lipid membranes.
机译：近年来，在促进对如何通过囊泡融合形成表面支撑膜的理解上花费了巨大的努力。目的是找到简单的模型系统，以研究细胞膜成分之间的生物物理和生化相互作用，例如，改变膜功能的药物和毒素。因此，设计和控制模型膜在传感器基底上的自组装构成了重要的研究领域，使其能够用于例如药物筛选，动态生物界面，人工鼻以及对膜活性抗生素的研究。作者已经开发并研究了带强负电荷的脂质膜的形成，该膜系统性地模仿了三种重要生物传感器材料上的细菌膜组成：SiO2，TiO2和氧化铟锡。通过平衡脂质囊泡电荷与Ca2 +作为融合促进剂的离子强度来调节静电相互作用，作者优化了自组装并获得了对脂质囊泡-表面相互作用细节的新见解。该结果对于将来由复杂脂质组合物制备的专用脂质膜表面涂层的开发和应用将是有用的。吸附过程的特征是石英微天平具有耗散监测，光波导光模能谱和光漂白后的荧光恢复，从而可以确定非平面负载脂质膜的形成。

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