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首页> 外文期刊>Biochemistry and Biophysics Reports >Global mapping of post-translational modifications on histone H3 variants in mouse testes
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Global mapping of post-translational modifications on histone H3 variants in mouse testes

机译：小鼠睾丸中组蛋白H3变体翻译后修饰的整体图

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Mass spectrometry (MS)-based characterization is important in proteomic research for verification of structural features and functional understanding of gene expression. Post-translational modifications (PTMs) such as methylation and acetylation have been reported to be associated with chromatin remodeling during spermatogenesis. Although antibody- and MS-based approaches have been applied for characterization of PTMs on H3 variants during spermatogenesis, variant-specific PTMs are still underexplored. We identified several lysine modifications in H3 variants, including testis-specific histone H3 (H3t), through their successful separation with MS-based strategy, based on differences in masses, retention times, and presence of immonium ions. Besides methylation and acetylation, we detected formylation as a novel PTM on H3 variants in mouse testes. These patterns were also observed in H3t. Our data provide high-throughput structural information about PTMs on H3 variants in mouse testes and show possible applications of this strategy in future proteomic studies on histone PTMs. Graphical abstract Display Omitted Highlights ? Various post-translational modifications in histone H3 variants were characterized in the mouse testes. ? We specifically identified similar modified patterns based on immonium ions. ? Novel modified lysines in testis-specific H3 histone, H3t, were verified. ? Our approach will be helpful for the discovery of other novel or specific modifications during spermatogenesis.
机译：基于质谱（MS）的表征在蛋白质组学研究中对于验证结构特征和对基因表达的功能理解非常重要。据报道翻译后修饰（PTM）如甲基化和乙酰化与精子发生过程中的染色质重塑有关。尽管在生精过程中基于抗体和质谱的方法已应用于表征H3变异体上的PTM，但仍未充分研究变异体特异性PTM。我们通过基于质量，保留时间和铵离子存在差异的成功分离，通过基于MS的策略成功分离出H3变体中的几种赖氨酸修饰，包括睾丸特异的组蛋白H3（H3t）。除了甲基化和乙酰化以外，我们还在小鼠睾丸中检测到甲酰化作为H3变体上的新型PTM。在H3t中也观察到了这些模式。我们的数据提供了有关小鼠睾丸中H3变体上PTM的高通量结构信息，并显示了该策略在组蛋白PTM的未来蛋白质组学研究中的可能应用。图形摘要显示省略的突出显示？在小鼠睾丸中表征了组蛋白H3变体的各种翻译后修饰。 ？我们专门确定了基于铵离子的类似修饰模式。 ？验证了睾丸特异性H3组蛋白H3t中新型修饰的赖氨酸。 ？我们的方法将有助于发现精子发生过程中其他新颖或特定的修饰。

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