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Peptide-Modified Zwitterionic Porous Hydrogels for Endothelial Cell and Vascular Engineering

机译：肽修饰的两性离子多孔水凝胶，用于内皮细胞和血管工程

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Hydrogels allow control of gel composition and mechanics, and permit incorporation of cells and a wide variety of molecules from nanoparticles to micromolecules. Peptide-linked hydrogels should tune the basic polymer into a more bioactive template to influence cellular activities. In this study, we first introduced the generation of 2D poly-(sulfobetaine methacrylate [SBMA]) hydrogel surfaces. By incorporating with functional peptide RGD and vascular endothelial growth factor-mimicking peptide KLTWQELYQLKYKG (QK) peptides, endothelial cells attached to the surface well and proliferated in a short-term culturing. However, the mechanical property, which plays a crucial role directing the cellular functions and supporting the structures, decreased when peptides graft onto hydrogels. Manipulating the mechanical property was thus necessary, and the most related factor was the monomer concentration. From our results, the higher amount of SBMA caused greater stiffness in hydrogels. Following the 2D surface studies, we fabricated 3D porous hydrogels for cell scaffolds by several methods. The salt/particle leaching method showed a more reliable way than gas-foaming method to fabricate homogeneous and open-interconnected pores within the hydrogel. Using the salt/particle leaching method, we can control the pore size before leaching. Morphology of endothelial cells within scaffolds was also investigated by scanning electron microscopy, and histological analysis was conducted in vitro and in vivo to test the biocompatibility of SB hydrogel and its potential as a therapeutic reagent for ischemic tissue repair in mice.
机译：水凝胶允许控制凝胶组成和力学，并允许细胞和从纳米颗粒到微分子的各种分子的结合。肽连接的水凝胶应将基础聚合物调整为更具生物活性的模板，以影响细胞活性。在这项研究中，我们首先介绍了2D聚（磺基甜菜碱甲基丙烯酸甲酯[SBMA]）水凝胶表面的生成。通过结合功能性肽RGD和模拟血管内皮生长因子的肽KLTWQELYQLKYKG（QK）肽，内皮细胞可以很好地附着于表面并在短期培养中增殖。然而，当肽接枝到水凝胶上时，机械性能降低，而机械性能在指导细胞功能和支持结构方面起着至关重要的作用。因此，必须控制机械性能，而最相关的因素是单体浓度。根据我们的结果，SBMA的含量越高，水凝胶的硬度越高。在2D表面研究之后，我们通过几种方法制造了用于细胞支架的3D多孔水凝胶。盐/颗粒浸出法显示出比气体发泡法更可靠的方法，可在水凝胶中制造均匀且开放的互连孔。使用盐/颗粒浸出方法，我们可以在浸出之前控制孔径。还通过扫描电子显微镜研究了支架内内皮细胞的形态，并在体外和体内进行了组织学分析，以测试SB水凝胶的生物相容性及其作为小鼠缺血性组织修复治疗剂的潜力。

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