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首页> 外文期刊>Journal of drug delivery science and technology >Injectable hydrogel delivering bone morphogenetic protein-2, vascular endothelial growth factor, and adipose-derived stem cells for vascularized bone tissue engineering
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Injectable hydrogel delivering bone morphogenetic protein-2, vascular endothelial growth factor, and adipose-derived stem cells for vascularized bone tissue engineering

机译：可注射水凝胶递送骨形态发生蛋白-2，血管内皮生长因子，以及用于血管化骨组织工程的脂肪衍生的干细胞

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We aimed to evaluate the integration of stem cells with injectable microspheres loaded with growth factors using a chitosan (CS) scaffold to promote healthy bone regeneration. We prepared and injected CS scaffolds containing adipose-derived stem cell (ADSC)-, vascular endothelial growth factor (VEGF)-, and bone morphogenetic protein-2 (BMP-2)-loaded nanohydroxyapatite (nHA)/poly lactic-co-glycolic acid microspheres (PLGAs) into critical-sized mandibular bone defects in rabbits. This injectable hydrogel displayed excellent physicochemical properties and degradability and achieved sustainable release and bioactivity preservation for both BMP-2 and VEGF. Computed tomography imaging and gross and histological observations indicated that bone defect cavities in the control group did not narrow and were filled with granulation tissue. Limited new bone formation for the first 12 weeks post-operation was observed in the defect cavities of the VEGF group; the scaffold degraded gradually and was replaced with fibrous connective tissue. In the BMP-2 or BMP-2/VEGF groups, bone defect cavities narrowed with time and healed completely after 12 weeks; scaffolds gradually degraded and infiltrated tightly into the surrounding tissues. Overall, the BMP-2/VEGF group demonstrated greater new bone formation, faster healing, and better callus remodeling. Simultaneous application of VEGF and BMP-2, therefore, promoted ossification and vascularization in critical-sized rabbit mandibular bone defects.
机译：我们旨在评估干细胞与使用壳聚糖（CS）支架的可注射微球的整合性微球，以促进健康的骨再生。我们制备和注射含有脂肪衍生的干细胞（ADSC） - ，血管内皮生长因子（VEGF） - 以及骨形态学蛋白-2（BMP-2） - 加载的纳米羟基磷灰石（NHA）/聚乳酸 - 共乙醛的Cs支架酸微球（PLGA）兔临界颌骨骨缺损。这种可注射水凝胶显示出优异的物理化学性质和可降解性，并实现了BMP-2和VEGF的可持续释放和生物活性保存。计算的断层摄影成像和总体和组织学观察表明对照组中的骨缺陷腔并未窄并且填充肉芽组织。在VEGF组的缺陷腔中观察到术后12周的新骨形成有限;支架逐渐降解并用纤维结缔组织替换。在BMP-2或BMP-2 / VEGF组中，骨缺损腔随时间变窄并在12周后完全愈合;支架逐渐降解并渗透紧密地进入周围组织。总体而言，BMP-2 / VEGF组展示了更大的骨形成，更快的愈合，更好的愈伤组织重塑。因此，同时施用VEGF和BMP-2，在临界大小的兔下颌骨缺陷中促进了骨化和血管化。

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