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首页> 外文期刊>BioResearch open access. >Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering
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Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering

机译：纤连蛋白在静电纺丝PLGA牙周组织工程支架上的表面截留

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Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly( l -lactide) (PLA), poly( d,l -lactide- co -glycolide) (PLGA), and poly-?-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01?M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10?μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01?M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.
机译：如今，组织工程领域的挑战在于开发旨在再生完整的受损组织的生物材料。尽管目前在软组织再生研究中使用生物可吸收聚酯，例如聚（l-丙交酯）（PLA），聚（d，l-丙交酯-共-乙交酯）（PLGA）和聚-ε-己内酯，但它们的疏水性负面影响细胞粘附。在这里，为了克服它，我们开发了一种纤连蛋白（FN）功能化的静电纺丝PLGA支架，用于牙周膜再生。电纺PLGA支架的功能化是通过碱水解（0.1或0.01？M NaOH）进行的。然后，通过简单沉积FN层（10？μg/ mL）来涂覆水解的支架。 X射线光电子分析证明了FN涂层。观察到接触角的减小和对水解的，FN包被的PLGA支架的更大细胞粘附性。在长达28天的时间内，所有样品均观察到了合适的降解行为而没有pH值变化。所有处理过的材料均表现出强收缩性，纤维取向损失和纤维塌陷。但是，与未处理的支架相比，使用0.01？M NaOH浓度进行功能化的过程会导致支架孔隙率保持不变，化学成分得以保留，并且机械性能相似。为电纺PLGA纤维功能化所提出的简化方法是使聚酯支架更具生物相容性的有效途径，并显示出组织工程的潜力。

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