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首页> 外文期刊>BioResearch open access. >Human Skin Cells That Express Stage-Specific Embryonic Antigen 3 Associate with Dermal Tissue Regeneration
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Human Skin Cells That Express Stage-Specific Embryonic Antigen 3 Associate with Dermal Tissue Regeneration

机译：表达阶段特异性胚胎抗原3与皮肤组织再生相关的人类皮肤细胞

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Stage-specific embryonic antigen 3 (SSEA3) is a glycosphingolipid that has previously been used to identify cells with stem cell-like, multipotent, and pluripotent characteristics. A rare subpopulation of SSEA3-expressing cells exists in the dermis of adult human skin. These SSEA3-expressing cells undergo a significant increase in cell number in response to injury, suggesting a possible role in regeneration. These SSEA3-expressing regeneration-associated (SERA) cells were derived through primary cell culture, purified by fluorescence-activated cell sorting (FACS), and characterized. Longer in vitro culture of the primary skin cells led to lower SSEA3 expression stability after FACS-based purification, suggesting that the current culture conditions may need to be optimized to permit the large-scale expansion of SERA cells. The SERA cells demonstrated a global transcriptional state that was most similar to bone marrow- and fat-derived mesenchymal stem cells (MSCs), and the highest expressing SSEA3-expressing cells co-expressed CD105 (clone 35). However, while a rare population of MSCs was observed in primary human skin cell cultures that could differentiate into adipocytes, osteoblasts, or chondrocytes, SERA cells did not possess this differentiation capacity, suggesting that there are at least two different rare subpopulations in adult human skin primary cultures. The identification, efficient purification, and large-scale expansion of these rare subpopulations (SERA cells and MSCs) from heterogeneous adult human skin primary cell cultures may have applications for future patient-specific cellular therapies.
机译：阶段特异性胚胎抗原3（SSEA3）是一种糖鞘脂，以前已用于鉴定具有干细胞样，多能和多能特性的细胞。成年人类皮肤的真皮中存在罕见的SSEA3表达细胞亚群。这些表达SSEA3的细胞响应损伤而细胞数量显着增加，提示其可能在再生中发挥作用。这些表达SSEA3的再生相关（SERA）细胞通过原代细胞培养获得，并通过荧光激活细胞分选（FACS）进行纯化和表征。基于FACS的纯化后，原代皮肤细胞的更长的体外培养导致较低的SSEA3表达稳定性，这表明可能需要优化当前的培养条件以允许SERA细胞的大规模扩增。 SERA细胞表现出与骨髓和脂肪来源的间充质干细胞（MSC）最相似的全局转录状态，而表达最高的SSEA3表达细胞共表达CD105（克隆35）。然而，尽管在人类原代皮肤细胞培养物中观察到稀有的MSC可以分化为脂肪细胞，成骨细胞或软骨细胞，但SERA细胞不具备这种分化能力，这表明在成年人类皮肤中至少有两个不同的稀有亚群原始文化。从异质成年人类皮肤原代细胞培养物中鉴定，有效纯化和大规模扩增这些稀有亚群（SERA细胞和MSC）可能会在未来的患者特异性细胞疗法中得到应用。

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