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首页> 外文期刊>Biosensors Journal >Blocking Non-Specific Binding for Phage-Based Magnetoelastic Biosensors
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Blocking Non-Specific Binding for Phage-Based Magnetoelastic Biosensors

机译:阻止基于噬菌体的磁弹性生物传感器的非特异性结合。

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The magnetoelastic (ME) biosensors are used to detect pathogen in fresh juice or milk by solenoid coil, and also developed for real-time, direct pathogen detection on food surfaces by surface-scanning coil. This paper presents blocking effect of different reagents on non-specific binding for detecting Salmonella typhimurium in apple juice using phage-based magnetoelastic biosensors. Three different blocking reagents of Bovine serum albumin, Superblock blocking buffer and blocker BLOTTO were used and evaluated. The results shows that blocker BLOTTO has the best blocking effect on non-specific binding.
机译:磁弹性(ME)生物传感器用于通过电磁线圈检测鲜榨果汁或牛奶中的病原体,还开发用于通过表面扫描线圈对食品表面进行实时,直接的病原体检测。本文介绍了基于噬菌体的磁弹性生物传感器检测苹果汁中鼠伤寒沙门氏菌时不同试剂对非特异性结合的阻断作用。使用并评估了三种不同的牛血清白蛋白封闭剂,Superblock封闭缓冲液和BLOTTO封闭剂。结果表明,阻滞剂BLOTTO对非特异性结合具有最佳的阻滞作用。

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