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Response of soil microflora to impact of heavy metals in zones of influence of railway transport

机译:铁路运输影响区土壤微生物区系对重金属影响的响应

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Monitoring research on the areas intensively and continuously affected by technogenic loading has remained topical until nowadays. The?soil as a basic component of many ecosystems, including the structure of its microbial cenoses, remains an informative index of a system’s overall stability. The areas affected by railway transport have been scarcely studied. Due to the above, the aim of this work has been to establish the transformation regularities of soil microbial cenoses of territories close to railways and to establish the groups of microorganisms that are a sensitive criterion of technogenic vehicular influence. For the purpose of microbiological research, soil samples were taken at different distances from the railway track (0, 25, 50, 100 and250 m) within five monitoring sections of the Tchop – Uzhhorod – Sambor railway (in the territory of Zakarpatska oblast). The number of ecological trophic groups was identified by means of inoculation on nutrient media using the method of serial dilution of soil suspensions. The research showed that in all types of soils that were adjacent to railway tracks, the number of ammonifiers and spore microbiota was high due to the high content of heavy metals (beyond the background levels). Besides, the bacterial microflora on beef-extract agar was characterized by homogeneity with domination of enteric bacteria and spore bacteria. Simultaneously, the numbers of nitrogen-fixing microorganisms, micromycetes, oligonitrophils, amylolytic and pedotrophic microflorae were shown to be low compared to the control. Farther from the railway track, pigmental species of bacteria appeared in the soil samples, attesting to the activity of self-purification processes. Correlation analysis of the data showed that the soil microbiota of the railway-side areas was undergoing changes as affected by heightened contents of heavy metals. Existence of medium and close connections was established between the number of microorganisms of ecological trophic groups and the content of acid-soluble lead forms at the levels of 0.72–1.72 maximum permissible concentrations. It was the nitrogen-fixing microorganisms and microscopic fungi that showed a fast response to heavy metals, which was an indication of their possible use as indicators of the ecological state of technogenically transformed soils.?.
机译:直到今天,对受技术负荷严重和持续影响的区域的监测研究仍是热门话题。土壤作为许多生态系统的基本组成部分,包括其微生物普查的结构,仍然是系统整体稳定性的有益指标。很少研究受铁路运输影响的地区。由于上述原因,这项工作的目的是建立靠近铁路的领土土壤微生物普查的转化规律,并建立作为技术性车辆影响敏感标准的微生物群。为了进行微生物学研究,在Tchop – Uzhhorod – Sambor铁路(位于Zakarpatska州的领土)的五个监测段内,从距铁轨(0、25、50、100和250 m)不同距离处采集了土壤样品。通过对土壤悬浮液进行系列稀释的方法,通过在营养培养基上接种来确定生态营养族的数量。研究表明,由于重金属含量高(超过本底水平),在与铁路轨道相邻的所有类型的土壤中,氨化菌和孢子菌群的数量都很高。此外,牛肉提取琼脂上的细菌菌群的特征是同质性,以肠杆菌和孢子菌为主。同时,与对照相比,固氮微生物,微霉菌,寡硝基亲子,淀粉分解菌和营养养分菌的数量显示较低。距离铁轨较远的地方,土壤样品中出现了细菌的色素物种,证明了自净化过程的活性。数据的相关分析表明,铁路侧区域的土壤微生物区系受到重金属含量增加的影响而发生变化。生态营养类微生物的数量与酸溶性铅形式的含量在最大允许浓度0.72–1.72之间建立了中度和紧密的联系。固氮微生物和微观真菌对重金属表现出快速响应,这表明它们可能被用作技术转化土壤生态状态的指标。

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