...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Complementary and Alternative Medicine >Isolation of anti-mycobacterial compounds from Curtisia dentata (Burm.f.) C.A.Sm (Curtisiaceae)
【24h】

Isolation of anti-mycobacterial compounds from Curtisia dentata (Burm.f.) C.A.Sm (Curtisiaceae)

机译：从齿叶Curtisia dentata（Burm.f.）C.A.Sm（Curtisiaceae）分离抗分枝杆菌化合物

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Tuberculosis is counted amongst the most infectious and lethal illnesses worldwide and remains one of the major threats to human health. The aim of the current study was to isolate and characterize anti-mycobacterial compounds present in Curtisia dentata (Burm.f.) C.A.Sm , a medicinal plant reportedly used in the treatment of tuberculosis, stomach ailments and sexually transmitted infections. Methods The bioassay guided principle was followed to isolate the anti-mycobacterial compounds. The crude ethanol extracts of the leaves was partitioned with various solvents four compounds such as β-sitosterol, betulinic acid, ursolic acid and lupeol were successfully isolated. The compounds and their derivatives were evaluated for anti-mycobacterial activity using Microplate Alamar Blue Assay (MABA) against Mycobacterium tuberculosis H37RV (ATCC 27294). Furthermore, the derivatives were investigated for their toxicity against HepG2 and HEK293 using the MTT assay. Results The methanol fraction had the lowest minimum inhibitory concentration (MIC) of 22.2?μg/ml against the selected Mycobacterium strain when compared to other fractions. Ursolic acid acetate (UAA) was the most active compound with MIC value of 3.4?μg/ml. The derivatives had varying degrees of toxicity, but were generally non-toxic to the selected cell lines. Derivatives also exhibited highest selectivity index and offers a higher safety margin. Conclusions The derivatives had better antimicrobial activity and low cytotoxic effects compared to isolated compounds. These increased their selectivity. It appears that acetylation of both betulinic acid and ursolic acid increased their activity against the selected Mycobacterium species. The results obtained in this study gives a clear indication that Curtisia dentata may serve as major source of new alternative medicines that may be used to treat TB. Furthermore, there is a need to explore the activity of these tested plant against other pathogenic Mycobacterium species.
机译：背景技术结核病被认为是世界上最具传染性和致命性的疾病之一，仍然是对人类健康的主要威胁之一。当前研究的目的是分离和鉴定齿状齿科（Burtis.f。）C.A.Sm中存在的抗分枝杆菌化合物，据报道该药用植物用于治疗结核病，胃病和性传播感染。方法遵循生物测定指导原则，分离出抗分枝杆菌化合物。叶子的粗乙醇提取物用各种溶剂分配，成功分离了四种化合物，如β-谷甾醇，桦木酸，熊果酸和羽扇豆酚。使用Microplate Alamar Blue Assay（MABA）对结核分枝杆菌H37RV（ATCC 27294）评估了化合物及其衍生物的抗分枝杆菌活性。此外，使用MTT分析研究了衍生物对HepG2和HEK293的毒性。结果与其他馏分相比，甲醇馏分对所选分枝杆菌菌株的最低最低抑菌浓度（MIC）为22.2μg/ ml。乙酸乌索酸（UAA）是活性最高的化合物，MIC值为3.4？g / ml。衍生物具有不同程度的毒性，但通常对所选细胞系无毒。衍生物还表现出最高的选择性指数，并提供更高的安全裕度。结论与分离的化合物相比，该衍生物具有更好的抗菌活性和较低的细胞毒性作用。这些增加了它们的选择性。看来，桦木酸和熊果酸的乙酰化都增强了它们对所选分枝杆菌种类的活性。这项研究获得的结果清楚地表明，牙本质病可能是可用于治疗结核病的新替代药物的主要来源。此外，需要探索这些测试植物对其他病原性分枝杆菌物种的活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号