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首页> 外文期刊>BioTechnology: An Indian Journal >Partition coefficient studies of calotropain from its crude latex using ionic liquid based aqueous two phase extraction system
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Partition coefficient studies of calotropain from its crude latex using ionic liquid based aqueous two phase extraction system

机译：离子液体基水相两相萃取系统研究促钙素从粗胶乳中的分配系数

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Separation and purification of high value bio-molecules using Ionic liquid (IL) based aqueous two phase (ATP) extraction is an emerging green separation process. IL based ATP system is the best alternative for the conventional organic solvent extraction and polymer basedATP systems. The current study is to compare the partition coefficient of calotrpin from the latex of medicinal plant Calotropis procera (Family-Asclepiadaceae; The molecular mass and iso-electric point of the enzyme are 28.8 kDa and 9.32, respectively) and the same was compared with conventional ATP system. The resulting protein was purified on Gel filtration chromatography column and it is confirmed with SDS page analysis. From the results it was found that IL based ATP system is highly selective and yield higher than the conventional ATP system.
机译：使用基于离子液体（IL）的水两相（ATP）萃取分离和纯化高价值生物分子是一种新兴的绿色分离方法。基于IL的ATP系统是常规有机溶剂萃取和基于聚合物的ATP系统的最佳替代方案。当前的研究是比较药用植物Calotropis procera（家族的乳杆菌科;该酶的分子质量和等电点分别为28.8 kDa和9.32）中calotrpin的分配系数，并将其与常规方法进行比较。 ATP系统。在凝胶过滤色谱柱上纯化所得蛋白质，并通过SDS页面分析确认。从结果发现，基于IL的ATP系统是高度选择性的，并且产率高于常规ATP系统。

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