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首页> 外文期刊>BioTechnology: An Indian Journal >Process optimization for human growth hormone biosynthesis by recombinant Escherichia coli
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Process optimization for human growth hormone biosynthesis by recombinant Escherichia coli

机译：重组大肠杆菌生物合成人生长激素的工艺优化

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摘要

Recombinant human growth hormone (r-hGH) can be produced by recombinant Escherichia coli a main host for recombinant protein productions. In order to improve the productivity of r-hGH, an orthogonal (L16(43×22)) experiment was applied to optimize the best culture conditions for r-hGH production by flask cultures of E. coli BL (21) harboring a new constructed plasmid (pEHUb-hGH). Based on the results of primary tests, five factors such as culture medium, culture volume, induction starting time, r-hGH expression time, and IPTG concentration were chosen for this present investigation as themain factors to influence the r-hGH expression. The optimum culture conditions were determined as follows: culture mediumas themodified TB, culture volume 50mL, induction starting time 3 h, r-hGHexpression time 7 h, and IPTG0.3mmol/L.Under this optimized conditions, the r-hGH productivity could reached 18.72 mg/L·h in flask culture. A high r-hGH concentration of 4.3 g/L, resulting in a high productivity of 215 mg/ L·h, could be obtained in the fed-batch culture of recombinant E. coli.
机译：重组人生长激素（r-hGH）可以通过重组大肠杆菌生产，而重组大肠杆菌是重组蛋白生产的主要宿主。为了提高r-hGH的生产率，通过正交实验（L16（43×22））优化了带有新构建的大肠杆菌BL（21）瓶培养的r-hGH生产的最佳培养条件。质粒（pEHUb-hGH）。根据初步测试结果，选择了培养基，培养液体积，诱导起始时间，r-hGH表达时间和IPTG浓度等五个因素作为影响r-hGH表达的主要因素。确定最佳培养条件为：以改良TB为培养基，培养体积为50mL，诱导起始时间为3 h，r-hGH表达时间为7 h，IPTG0.3mmol / L。在此优化条件下，r-hGH的产量可达烧瓶培养中为18.72 mg / L·h。在重组大肠杆菌的分批分批培养中，可以达到4.3 g / L的高r-hGH浓度，导致215 mg / L·h的高生产率。

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