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首页> 外文期刊>BioTechnology: An Indian Journal >Application of response surface methodology for poly-§-glutamic acid production by Bacillus subtilis ATCC 6633
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Application of response surface methodology for poly-§-glutamic acid production by Bacillus subtilis ATCC 6633

机译：响应面法在枯草芽孢杆菌ATCC 6633生产聚§-谷氨酸中的应用

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摘要

The objective of this study was to investigate the production of poly-???§- glutamic acid (???§-PGA) by Bacillus subtilisATCC 6633, using surface response methodology. Two successive factorial designs were used to determine the sucrose concentration, addition of glutamic acid and time of culture in fermentationmediumfor the production of ???§-PGA. The results of the statistical 23 full factorial design indicated that the linear terms, sucrose concentration, addition of glutamic acid and fermentation time, were significant and positive for the production of the biopolymer. Based on these results, a central composite design was utilized in order to optimize the production of ???§-PGA and define the best concentrations of sucrose and glutamic acid and fermentation time. The best production of ???§-PGA was 20.4 g/l obtained with 132 g/l sucrose, 27.6 g/l glutamic acid and 104 h of fermentation, and the productivity for the maximal production of ???§-PGA was 0.1962 g/l.h-1.
机译：这项研究的目的是研究枯草芽孢杆菌ATCC 6633使用表面反应方法生产聚β-谷氨酸（β-PGA）的方法。用两个连续的因子设计来确定蔗糖浓度，谷氨酸的添加量和发酵培养基中培养β-PGA的时间。统计的23个全因子设计的结果表明，线性项，蔗糖浓度，谷氨酸的添加量和发酵时间对生物聚合物的生产是重要的并且是积极的。基于这些结果，为了优化β-PGA的生产并确定蔗糖和谷氨酸的最佳浓度和发酵时间，采用了中央复合设计。 §-PGA的最佳产量是用132g / l的蔗糖，27.6g / l的谷氨酸和104小时的发酵得到的20.4g / l，最大产量的§-PGA为： 0.1962 g / lh-1。

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