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首页> 外文期刊>BioTechnology: An Indian Journal >Enhancement of 2,3-butanediol production by Klebsiella oxytoca PTCC 1402
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Enhancement of 2,3-butanediol production by Klebsiella oxytoca PTCC 1402

机译：产酸克雷伯氏菌PTCC 1402增强2,3-丁二醇的生产

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In this study, optimal operating parameters of 2,3-Butanediol production using Klebsiell aoxytoca under submerged culture conditions are determined by using Taguchi method. The effect of different factors including medium composition, pH, temperature, mixing intensity and inoculum size on 2,3-butanediol production was analyzed using the Taguchi method in three levels. Based on these analyses the optimum concentrations of glucose, acetic acid and succinic acid were found to be 6, 0.5 and 1.0 (%w/v) respectively. Furthermore, optimum values for temperature, inoculumsize, pH and the shaking speed were determined as 37???°C, 8 (g/L), 6.1 and 150 rpmrespectively. The optimized conditions showed an enhanced 2,3-Butanediol yield of 40%[from0.539 to 0.897 (g/g)]. The optimal combinations of factors obtained from the proposed DOE methodology was further validated by conducting fermentation experiments and the obtained results revealed an enhanced 2,3-Butanediol yield of 27.63%.
机译：在这项研究中，通过田口方法确定了在水下培养条件下使用无氧克雷伯生产2,3-丁二醇的最佳操作参数。使用Taguchi方法从三个层面分析了培养基成分，pH，温度，混合强度和接种量等不同因素对2,3-丁二醇产量的影响。基于这些分析，发现葡萄糖，乙酸和琥珀酸的最佳浓度分别为6、0.5和1.0（％w / v）。此外，确定温度，接种量，pH和振荡速度的最佳值分别为37℃，8（g / L），6.1和150rpm。优化的条件显示2,3-丁二醇产率提高了40％[从0.539到0.897（g / g）]。通过进行发酵实验进一步验证了从提出的DOE方法获得的因子的最佳组合，获得的结果表明提高了2,3-丁二醇收率27.63％。

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