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首页> 外文期刊>BioTechnology: An Indian Journal >Antimethanogenic activity of essential oils extracted from Rosmarinus officinalis and Lavandula officinalis
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Antimethanogenic activity of essential oils extracted from Rosmarinus officinalis and Lavandula officinalis

机译：迷迭香和薰衣草精油的促孕激素作用。

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Methane constitutes the main way of hydrogen elimination in the rumen during the microbial digestion of food. Its eructation by ruminants led to both energy loss for animals and a worsening of the greenhouse by its radioactive power. The aimof this study focuses on exploring the ability of essential oils (EO), extracted fromrosemary (Rosmarinus officinalis) and lavender (Lavandula officinalis), in reducing ruminalmethanogenesis.Activity of essential oils extractswas compared to that of purified essential oils (carvacrol, cinnamaldehyde and thymol). The aerial parts of plants (rosemary and lavender) are used fresh. Extraction of EO was made by the hydrodistillation process.Antimethanogenic activity of EO extracts was measured in vitro in batch systems (polypropylene syringes, 60 ml capacity). EO extract of each plant and purified EO (carvacrol, cinnamaldehyde and thymol) were tested at different doses: 10, 20 and 40 ìl. Gas production was monitored at different time intervals: 2, 4, 6, 8, 24, 48 and 72 h. The qualitative and quantitative analysis of fermentative gas (CH4 and CO2) was chemically determinate after 24 hours incubation. The results indicate that addition of crude oils of Rosmarinus officinalis and Lavandula officinalis and the purified oils did not affect significantly the pH values (P > 0.05). The addition of EO of both plants did not affect gas production after 2 hours of fermentation (P > 0.05) for the three doses. Between 4 and 6 h of incubation, increasing EO dose of Lavandula officinalis induces a continuous decline (but not significant) of the gas production compared with the control (no additives). Similarly, addition of Rosmarinus officinalis essential oils had not significant effect on gas production for 10 and 40 ìl doses compared with control. However, higher gas productionwas recorded for 20ìl dose. Addition of Lavandula officinalis EOat 40μl level caused significant decrease in gas production after 24h incubation (P < 0.05). Methane production was significantly reduced in the presence of EO extracted fromRosmarinus officinalis (28.1%; P < 0.05). Besides, EOextracted fromLavendula officinalis decrease significantlymethane production during 24 hours of incubation for 10 and 40ìl doses. In general, EO used in our study showed a significant effect on reducing methane. Thus, we can focus future research on composition characterization of each essential (CG???MS) and to determine minimal inhibitory concentration (MIC) allows maximum methane reduction without adverse effects on digestibility.
机译：甲烷是食品微生物消化过程中瘤胃中除氢的主要途径。反刍动物对它的引诱既导致动物的能量损失，又由于其放射性导致温室的恶化。本研究的目的在于探索从迷迭香（Rosmarinus officinalis）和熏衣草（Lavandula officinalis）中提取的精油（EO）减少瘤胃甲烷生成的能力。与精制精油（香芹酚，肉桂醛和百里香酚）。植物的地上部分（迷迭香和薰衣草）新鲜使用。 EO的提取通过加氢蒸馏过程进行.EO提取物的产甲烷活性在体外分批系统（聚丙烯注射器，容量为60 ml）中进行了测量。测试了每种植物的EO提取物和纯化的EO（香芹酚，肉桂醛和百里酚）的不同剂量：10、20和40μl。在不同的时间间隔（2、4、6、8、24、48和72小时）监测产气量。孵育24小时后，化学测定了发酵气体（CH4和CO2）的定性和定量分析。结果表明，添加迷迭香和厚朴的原油以及纯化的油不会显着影响pH值（P> 0.05）。三种剂量的发酵2小时后（P> 0.05），两种植物中EO的添加均不影响产气量。在孵育的4至6小时之间，与对照（无添加剂）相比，熏衣草的EO剂量增加会导致气体产量的连续下降（但不显着）。同样，与对照相比，添加迷迭香精油对10和40 µl剂量的产气量也没有显着影响。但是，记录到20升剂量会产生更高的产气量。孵育24小时后，添加薰衣草EOat40μl水平会显着降低产气量（P <0.05）。在从迷迭香中提取的EO存在下，甲烷的产生显着降低（28.1％; P <0.05）。此外，从薰衣草中提取的EO在培养10和40μl剂量的24小时内可显着降低甲烷生成。通常，在我们的研究中使用的EO对减少甲烷有明显的作用。因此，我们可以将未来的研究重点放在每种必需成分的组成表征（CG-MS）上，并确定最小抑制浓度（MIC）可以最大程度地减少甲烷，而不会对消化率产生不利影响。

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