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首页> 外文期刊>Biotechnology for Biofuels >On-farm solid state simultaneous saccharification and fermentation of whole crop forage rice in wrapped round bale for ethanol production
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On-farm solid state simultaneous saccharification and fermentation of whole crop forage rice in wrapped round bale for ethanol production

机译：整捆草捆中全农用饲料稻米的固态固态糖化和发酵用于乙醇生产

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Background In an attempt to reduce environmental loading during ethanol production from cellulosic plant biomass, we have previously proposed an on-site solid state fermentation (SSF) method for producing ethanol from whole crops, which at the same time provides cattle feed without producing wastes. During the ensiling of freshly harvested plant biomass with cellulase and glucoamylase, the added yeast and lactic acid bacteria induced simultaneous saccharification and production of ethanol and lactic acid in hermetically sealed containers on-farm. In a previous study, laboratory-scale SSF (using 250 g of fresh rice crop biomass) yielded 16.9 weight % ethanol in dry matter (DM) after 20 days of incubation. In this study, the fermentation volume was scaled up to a normal-sized round bale and the fermentation process (ethanol concentrations of the products) was monitored. The ethanol produced was recovered and the recovery efficiency was evaluated. Results SSF tests with forage rice round bales using polyethylene-wrapped whole plant materials (cultivar Leaf Star, average of 125.2 kg dry weight) were monitored in the field without temperature control. They yielded 14.0 weight % ethanol and 2.9 weight % lactic acid in DM after six months of incubation, and the ethanol ratio in the bale remained stable for 14 months after processing. SSF tests with three different rice cultivars were conducted for three years. Ethanol recovery from a fermented whole bale (244 kg fresh matter (FM) containing about 12.4 kg ethanol) by one-step distillation using vacuum distillation equipment yielded 86.3% ethanol collected from distilled solution (107 kg of 10.0 weight % ethanol). In addition, an average of 1.65 kg ethanol in 40.8 kg effluent per bale was recovered. Relative nitrogen content was higher in SSF products than in silage made from the same plant material, indicating that fermentation residue, whose quality is stabilized by the lactic acid produced, can be used as cattle feed. Conclusions We have successfully demonstrated an efficient on-site ethanol production system with non-sterilized whole rice crop round bale. However, issues concerning the establishment of the ethanol recovery procedure on-site and evaluation of the fermentation residue as cattle feed have to be addressed.
机译：背景技术为了减少纤维素植物生物质生产乙醇过程中的环境负荷，我们先前提出了一种现场固态发酵（SSF）方法，用于从整个农作物生产乙醇，该方法同时提供牛饲料而不产生废物。在用纤维素酶和葡糖淀粉酶包裹新鲜收获的植物生物量的过程中，添加的酵母菌和乳酸菌在农场的密闭容器中同时糖化并产生乙醇和乳酸。在先前的研究中，实验室规模的SSF（使用250克新鲜的水稻作物生物质）经过20天的培养后，在干物质（DM）中产生16.9重量％的乙醇。在这项研究中，发酵量按比例放大至正常大小的圆包，并监测发酵过程（产品的乙醇浓度）。回收产生的乙醇并评估回收效率。结果在没有温度控制的情况下，使用聚乙烯包裹的整株植物材料（栽培品种Leaf Star，平均干重125.2 kg）对草料大米圆捆进行SSF测试。孵育六个月后，它们在DM中产生14.0重量％的乙醇和2.9重量％的乳酸，处理后的14个月中，草捆中的乙醇比率保持稳定。用三种不同的水稻品种进行了SSF测试，为期三年。使用真空蒸馏设备通过一步蒸馏从发酵的整个棉包（244千克的新鲜物质（FM），约含12.4千克乙醇）中回收乙醇，从蒸馏溶液中收集到86.3％的乙醇（107千克的10.0重量％乙醇）。此外，每包40.8千克废液中平均回收了1.65千克乙醇。 SSF产品中的相对氮含量高于用相同植物原料制成的青贮饲料，这表明发酵残渣可以用作牛饲料，发酵残渣的质量由所产生的乳酸稳定。结论我们已经成功地展示了一种高效的现场乙醇生产系统，该系统具有未灭菌的整粒稻米圆捆草。但是，必须解决有关现场建立乙醇回收程序和评估发酵残渣作为牛饲料的问题。

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