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Sophora japonica extracts accelerates keratinocyte differentiation through miR-181a

机译：槐花提取物通过miR-181a促进角质形成细胞分化

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The Sophora japonica extracts contain flavonol triglycoside, isoflavonol, coumarone chromone, saponin, triterpene glucoside, phospholipids, alkaloids, amino acids, polysaccharides, and fatty acids. These components have physiological effects such as anti-infertility and anti-cancer activities. This study investigated the regulation of keratinocyte differentiation upon treatment with the S. japonica extracts in keratinocyte and the molecular cell biological mechanism involved. To determine whether the S. japonica extracts or troxerutin, which is its main component, regulates keratinocyte differentiation, quantitative real-time polymerase chain reaction (qRT-PCR) was performed on keratinocyte differentiation markers such as keratin 1 (K1), keratin 10 (K10), involucrin, and filaggrin after treatment with the S. japonica extracts. miR-181a knockdown confirmed that keratinocyte differentiation was regulated by increased miR-181a expression upon treatment with the S. japonica extracts or troxerutin. The expression of keratinocyte differentiation markers such as K1, K10, involucrin, and filaggrin increased upon treatment with the S. japonica extracts and troxerutin. Furthermore, miR-181a expression, which is known to increase during keratinocyte differentiation, increased upon treatment with the S. japonica extracts and troxerutin. When miR-181a was knocked down, the increased expression of keratinocyte differentiation markers upon treatment with the S. japonica extracts and troxerutin decreased again. Finally, it was confirmed that miR-181a directly regulated and reduced the expression of Notch2, which reduces keratinocyte differentiation, and that the decrease in Notch2 expression by miR-181a regulated keratinocyte differentiation. These results suggest that the S. japonica extracts or troxerutin accelerates keratinocyte differentiation through miR-181a. This accelerated keratinocyte differentiation was confirmed to have resulted from the regulation of Notch2 expression by miR-181a. The results of this study provide an opportunity to confirm the molecular cell biological mechanism of S. japonica extracts or troxerutin on skin keratinization, and we expected that this study contribute to develop a moisturizing cosmetic material that can strengthen the skin barrier through regulating keratinocyte differentiation.
机译：槐花提取物含有黄酮醇三糖苷，异黄酮醇，香豆酮色酮，皂苷，三萜糖苷，磷脂，生物碱，氨基酸，多糖和脂肪酸。这些成分具有生理作用，例如抗不孕和抗癌活性。这项研究调查了角质形成细胞中日本血吸虫提取物处理后角质形成细胞分化的调控及其涉及的分子细胞生物学机制。为了确定其主要成分是日本葡萄球菌提取物或曲克芦丁是否调节角质形成细胞的分化，对角质形成细胞分化标记物（例如角蛋白1（K1），角蛋白10（ S.japonica提取物处理后的K10），总蛋白和丝聚蛋白。 miR-181a敲低证实，在用日本血吸虫提取物或曲克芦丁处理后，miR-181a表达增加可调节角质形成细胞的分化。用日本血吸虫提取物和曲克芦丁处理后，角质形成细胞分化标记物（如K1，K10，整合蛋白和丝聚蛋白）的表达增加。此外，已知在角质形成细胞分化过程中会增加的miR-181a表达，在用日本血吸虫提取物和曲妥菌素处理后会增加。当将miR-181a敲低时，用日本血吸虫提取物和曲格鲁汀处理后，角质形成细胞分化标记物的表达增加。最后，证实了miR-181a直接调节并减少了Notch2的表达，这降低了角质形成细胞的分化，并且证实了miR-181a引起的Notch2表达的降低调节了角质形成细胞的分化。这些结果表明，日本粳稻提取物或曲格鲁汀通过miR-181a促进角质形成细胞分化。证实该加速的角质形成细胞分化是由miR-181a调节Notch2表达引起的。这项研究的结果提供了一个机会，可以确认日本刺槐提取物或曲克芦丁对皮肤角质化的分子细胞生物学机制，并且我们希望这项研究有助于开发一种保湿化妆品，该化妆品可以通过调节角质形成细胞的分化来增强皮肤屏障。

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