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首页> 外文期刊>BMC Biotechnology >Biosynthesis of trans-4-hydroxyproline by recombinant strains of Corynebacterium glutamicum and Escherichia coli
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Biosynthesis of trans-4-hydroxyproline by recombinant strains of Corynebacterium glutamicum and Escherichia coli

机译：谷氨酸棒杆菌和大肠杆菌的重组菌株对反式-4-羟脯氨酸的生物合成

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Background Trans- 4-hydroxy-L-proline ( trans -Hyp), one of the hydroxyproline (Hyp) isomers, is a useful chiral building block in the production of many pharmaceuticals. Although there are some natural biosynthetic pathways of trans -Hyp existing in microorganisms, the yield is still too low to be scaled up for industrial applications. Until now the production of trans -Hyp is mainly from the acid hydrolysis of collagen. Due to the increasing environmental concerns on those severe chemical processes and complicated downstream separation, it is essential to explore some environment-friendly processes such as constructing new recombinant strains to develop efficient process for trans -Hyp production. Result In this study, the genes of trans- proline 4-hydroxylase ( trans- P4H) from diverse resources were cloned and expressed in Corynebacterium glutamicum and Escherichia coli , respectively. The trans -Hyp production by these recombinant strains was investigated. The results showed that all the genes from different resources had been expressed actively. Both the recombinant C. glutamicum and E. coli strains could produce trans -Hyp in the absence of proline and 2-oxoglutarate. Conclusions The whole cell microbial systems for trans -Hyp production have been successfully constructed by introducing trans- P4H into C. glutamicum and E. coli . Although the highest yield was obtained in recombinant E. coli , using recombinant C. glutamicum strains to produce trans -Hyp was a new attempt.
机译：背景技术反式4-羟基-L-脯氨酸（反式-Hyp）是羟基脯氨酸（Hyp）异构体之一，是许多药物生产中有用的手性结构单元。尽管微生物中存在一些天然的反式-Hyp生物合成途径，但收率仍然太低，无法用于工业应用。到目前为止，反式-Hyp的生产主要来自胶原蛋白的酸水解。由于对那些严峻的化学过程和复杂的下游分离日益增加的环境关注，因此有必要探索一些环境友好的过程，例如构建新的重组菌株以开发有效的反式-Hyp生产方法。结果本研究从谷氨酸棒杆菌和大肠杆菌中克隆并表达了来自多种资源的脯氨酸4-羟化酶（trans-P4H）基因。研究了这些重组菌株的反式-Hyp产生。结果表明，来自不同资源的所有基因均得到了有效表达。在脯氨酸和2-氧戊二酸不存在的情况下，重组谷氨酸棒状杆菌和大肠杆菌菌株均可产生反式-Hyp。结论将反式P4H导入谷氨酸棒杆菌和大肠杆菌中已成功构建了反式-Hyp生产的全细胞微生物系统。尽管在重组大肠杆菌中获得最高产量，但使用重组谷氨酸棒杆菌菌株生产反式-Hyp却是一项新尝试。

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