• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BioTechnology: An Indian Journal >Phospholipase d triggers a high antibody response against acinetobacterbaumanii infection in murine model
【24h】

Phospholipase d triggers a high antibody response against acinetobacterbaumanii infection in murine model

机译：磷脂酶D在鼠模型中引发针对不动杆菌感染的高抗体反应

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Acinetobacterbaumannii, a major threatfor hospitalized patients, intensive care units (ICUs) residents, military forces, is a public health problem due to increasing rate of antibiotic resistance. Vaccination of these risk groups may lessen occurrence and spread of infections. In the present study efficiency of phospholipase D (PLD), one of previously identi?ed virulence factors of A. baumannii, was evaluated as a vaccine component. Polymerase chain reaction (PCR) was used to verify the presence of PLD virulence gene in clinical isolates. The pld was amplified, cloned and the recombinant protein i.e. rPLD, was expressed. BALB/C mice were immunized with the recombinant PLD followed by intraperitoneal challenge with lethal doses of 108 to 1011 CFU of A. baumannii. Experimental findings showed significantly higher average survival rate of 95% in immunized mice than the control group. Rabbits were immunized with the rPLD and the sera were used to determine its efficiency in protecting unimmunized mice from bacterial challenge.Average survival rate of 80%was achieved in challenged groups with the same lethal doses used in active immunization challenges compared with the control groups receiving naive rabbit serum. rPLD was potentially effective in triggering a high antibody response and protectingmice against A. baumannii.
机译：鲍曼不动杆菌是住院患者，重症监护病房（ICU）居民，军队的主要威胁，由于抗生素耐药性的增加，这是一个公共卫生问题。这些风险人群的疫苗接种可以减少感染的发生和传播。在本研究中，磷脂酶D（PLD）的效率已被评估为疫苗成分，而磷脂酶D是先前鉴定的鲍曼不动杆菌的毒力因子之一。聚合酶链反应（PCR）用于验证临床分离物中PLD毒力基因的存在。将pld扩增，克隆并表达重组蛋白即r​​PLD。用重组PLD免疫BALB / C小鼠，然后用致死剂量为鲍曼不动杆菌的108至1011 CFU的致死剂量腹膜内攻击。实验结果表明，免疫小鼠的平均存活率明显高于对照组，为95％。用rPLD免疫兔子，并使用血清确定其保护未免疫小鼠免受细菌攻击的效率。与接受免疫接种的对照组相比，在主动免疫攻击中使用相同致死剂量的挑战组中，平均存活率达到80％幼稚的兔血清。 rPLD在引发高抗体应答和保护小鼠免受鲍曼不动杆菌方面可能有效。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号