Various growth factor yields in various platelet rich plasma processing methods

Puji Sari; Evah Luviah; Yudhi Nugraha; Jeanne Adiwinata Pawitan; Jinia Lilianty; Reza Yuridian Purwoko

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Various growth factor yields in various platelet rich plasma processing methods

机译：各种富含血小板的血浆处理方法中各种生长因子的产量

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Aim: To compare the growth factor contents of the various PRP processing methods to get an insight on the difference in growth factor yield from the various processing methods. Experimental: PRP before and after expiry dates was collected from Indonesian Red Cross. PRP was processed by various methods to release the growth factors, and growth factor levels were measured. Growth factor levels between various kinds of processing were compared usingANOVAand those frombefore and after expiry date samples were compared using t test. Results: Comparison of thrombin activation, one, two, and three freeze thawcycle(s) of all samples revealed no significantdifference inPDGF-AB,EGF, and IGFlevels (p>0.05).However, there were significant differences in TGFa and VEGF levels between thrombin activation and freeze thawcycles. Comparisons between samples of before and after expiry date revealed no significant differences in all growth factor levels. Conclusion: One, two, and three freeze thawcycle(s) yielded no significant difference in most growth factor levels.

机译：目的：比较各种PRP加工方法中生长因子的含量，以了解各种加工方法中生长因子产量的差异。实验性：有效期之前和之后的PRP是从印尼红十字会收集的。通过各种方法处理PRP以释放生长因子，并测量生长因子水平。使用方差分析比较各种加工之间的生长因子水平，并使用t检验比较有效期前后样品的生长因子水平。结果：比较所有样本的凝血酶活化，1、2和3个冻融循环，发现PDGF-AB，EGF和IGF水平无显着差异（p> 0.05），但TGFa和VEGF水平之间存在显着差异凝血酶活化和冻融循环。过期前后样品之间的比较显示，所有生长因子水平均无显着差异。结论：一，二和三个冷冻解冻循环在大多数生长因子水平上没有显着差异。

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《BioTechnology: An Indian Journal》 |2014年第11期|共4页
作者
Puji Sari; Evah Luviah; Yudhi Nugraha; Jeanne Adiwinata Pawitan; Jinia Lilianty; Reza Yuridian Purwoko;
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中图分类生物工程学（生物技术）;
关键词
Thrombocyte concentratePlatelet rich plasmaGrowth factorsFreeze-thawThrombin activated;

机译：血小板浓缩液富含血小板的血浆生长因子冻融凝血酶激活;

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1. Optimal activation methods for maximizing the concentrations of platelet-derived growth factor-BB and transforming growth factor-β1 in equine platelet-rich plasma [J] . Kentaro FUKUDA, Taisuke KURODA, Norihisa TAMURA, The Journal of Veterinary Medical Science . 2020,第10期

机译：最大化血小板衍生生长因子-BB浓度和转化生长因子-β1在马血小板血小板血浆中的最佳活化方法
2. Shedding light in the controversial terminology for platelet-rich products: platelet-rich plasma (PRP), platelet-rich fibrin (PRF), platelet-leukocyte gel (PLG), preparation rich in growth factors (PRGF), classification and commercialism. [J] . Ehrenfest DM, Bielecki T, Del Corso M, Journal of biomedical materials research, Part A . 2010,第4期

机译：富血小板产品的术语引起争议：富血小板血浆（PRP），富血小板纤维蛋白（PRF），血小板白细胞凝胶（PLG），富含生长因子的制剂（PRGF），分类和商业化。
3. Comparison of the platelet concentrate collection system with the plasma-rich-in-growth-factors kit to produce platelet-rich plasma: a technical report. [J] . Weibrich G, Kleis WK, Hitzler WE, The International journal of oral & maxillofacial implants . 2005,第1期

机译：浓缩血小板收集系统与富含血浆的生长因子试剂盒生产富含血小板的血浆的比较：技术报告。
4. Effect of Freeze-Drying Process on Collagen-Activated Platelet-Rich Plasma into Platelet Derived Growth Factor-AB Level [C] . Kwartarini Murdiastuti, Fitri Yuniawati, Nunuk Purwanti, International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering . 2019

机译：冷冻干燥过程对胶原活化的血小板血浆中血小板衍生生长因子-AB水平的影响
5. Studies on Equine Platelet-Rich Plasma: Preparation and Activation Methods, Composition, In Vivo Effects, and Material Properties. [D] . Textor, Jamie A. 2012

机译：马富含血小板血浆的研究：制备和活化方法，组成，体内作用和材料特性。
6. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP) plasma rich in growth factors (PRGF) advanced platelet-rich fibrin (A-PRF) and concentrated growth factors (CGF) [O] . Hideo Masuki, Toshimitsu Okudera, Taisuke Watanebe, 2016

机译：富含血小板的血浆（PRP）富含生长因子（PRGF）晚期富含血小板的纤维蛋白（A-PRF）和浓缩生长因子（CGF）的生长因子和促炎细胞因子含量
7. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF) [O] . 2016

机译：富含血小板的血浆（PRP），富含生长因子（PRGF），晚期富含血小板的纤维蛋白（A-PRF）和浓缩生长因子（CGF）的生长因子和促炎细胞因子含量

Various growth factor yields in various platelet rich plasma processing methods

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