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首页> 外文期刊>BioTechnology: An Indian Journal >Seminal xanthine oxidase: Appropriate fluorometric assay for the examination of spermatozoa disorders
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Seminal xanthine oxidase: Appropriate fluorometric assay for the examination of spermatozoa disorders

机译：精子黄嘌呤氧化酶：用于检查精子疾病的适当荧光测定法

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摘要

A Fluorometric assay for semen xanthine oxidase activity that could be used as a back-up to current tests in the differential diagnosis of spermatozoa disorders is described. The assay is based on the H2O2- dependent oxidation of thiamine catalyzed by peroxidasemimetic (amixture of hematin (HT) and hemoglobin (HB)). The method is sensitive, precise (CV below 7.9%), and linear up to 40 U/l. The analytical recovery of the present method is estimated. The comparison with the UV method gave good correlation (r = 0.994). Themethod is applied to the measurement of the XO activity in seminal plasma of fertile men and Asthenzoospermic patients. Reference values for seminal xanthine oxidase activities determined with the presentmethod on 30 healthy persons are 70.37±23.1.
机译：描述了一种用于精液黄嘌呤氧化酶活性的荧光测定法，该测定法可作为对精子疾病鉴别诊断中当前测试的支持。该测定基于过氧化物酶模拟物（血红素（HT）和血红蛋白（HB）的混合物）催化的硫胺素的H 2 O 2依赖性氧化。该方法灵敏，精确（CV低于7.9％）并且线性高达40 U / l。估计本方法的分析回收率。与紫外线法的比较显示出良好的相关性（r = 0.994）。该方法用于测量可育男性和弱精子症患者精浆中的XO活性。用本方法测定的30名健康人的精子黄嘌呤氧化酶活性的参考值为70.37±23.1。

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